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Neuromedin B Monoclonal antibody

Neuromedin B Monoclonal Antibody for IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse and More (1)

Applications

IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G3B6

Cat no : 66781-1-Ig

Print datasheet

Synonyms

neuromedin B, NMB

验证数据展示

  • IHC staining of human gliomas using 66781-1-Ig

    IHC staining of human gliomas using 66781-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66781-1-Ig (NMB antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 66781-1-Ig

    IHC staining of human gliomas using 66781-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66781-1-Ig (NMB antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inhuman gliomas tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

66781-1-Ig targets Neuromedin B in IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity Human, mouse
Cited Reactivityhuman
Immunogen Neuromedin B fusion protein Ag0842 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name neuromedin B
Synonyms neuromedin B, NMB
Calculated Molecular Weight 16 kDa
GenBank Accession NumberBC007407
Gene Symbol NMB
Gene ID (NCBI) 4828
RRIDAB_2882126
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP08949
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neuromedin B (NMB) is a bombesin-like peptide, which inhibits food intake and modulates stress-related behaviour. NMB is encoded in a 76-amino acid precursor. The mature NMB was reported to be a 10-amino-acid peptide, giving two bands of 1.2 kDa and 1.5 kDa in western blotting. NMB is expressed in pituitary gland, hypothalamus, pancreas and stomach. It regulates exocrine and endocrine secretions, cell growth, body temperature and blood pressure and glucose level in vivo. (PMID: 15528253, PMID: 2458345).

实验方案

Product Specific Protocols
IHC protocol for Neuromedin B antibody 66781-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Ann N Y Acad Sci

The von Economo neurons in the frontoinsular and anterior cingulate cortex.

Authors - John M Allman
View Article