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Neuropilin 2 Polyclonal antibody

Neuropilin 2 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 27193-1-AP

Print datasheet

Synonyms

NRP2, Neuropilin-2

验证数据展示

  • WB analysis of U-87 MG using 27193-1-AP

    WB analysis of U-87 MG using 27193-1-AP

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 27193-1-AP (Neuropilin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of U-87 MG using 27193-1-AP

    IP experiment of U-87 MG using 27193-1-AP

    IP result of anti-Neuropilin 2 (IP:27193-1-AP, 4ug; Detection:27193-1-AP 1:500) with U-87 MG cells lysate 760 ug.

  • IHC staining of human intrahepatic cholangiocarcinoma using 27193-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 27193-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27193-1-AP (Neuropilin 2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 27193-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 27193-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27193-1-AP (Neuropilin 2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inU-87 MG cells
Positive IP detected inU-87 MG cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27193-1-AP targets Neuropilin 2 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Tested Reactivity human
Immunogen Neuropilin 2 fusion protein Ag25622 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name neuropilin 2
Synonyms NRP2, Neuropilin-2
Observed Molecular Weight120-130 kDa
GenBank Accession NumberBC104770
Gene Symbol Neuropilin 2
Gene ID (NCBI) 8828
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60462
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neuropilin 2 (NRP2) is a single-chain transmembrane glycoprotein that acts as a co-receptor of VEGF. NRP2 is mainly expressed in lymphatic and venous endothelial cells, and it is associated with the occurrence and development of several types of cancer. NRP2 is known to play a role in cardiovascular development, axon guidance, tumorigenesis, inflammation and cardiovascular disease.

实验方案

Product Specific Protocols
WB protocol for Neuropilin 2 antibody 27193-1-APDownload protocol
IHC protocol for Neuropilin 2 antibody 27193-1-APDownload protocol
IP protocol for Neuropilin 2 antibody 27193-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols