验证数据展示
经过测试的应用
| Positive WB detected in | mouse lung tissue, mouse uterus tissue |
| Positive IHC detected in | mouse spleen tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83102-1-RR targets Lipocalin-2/NGAL in WB, IHC, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | mouse |
| 免疫原 | Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | lipocalin 2 |
| 别名 | Lcn2, Lipocalin-2, Oncogene 24p3, Neutrophil gelatinase-associated lipocalin, Lipocalin 2 |
| 计算分子量 | 23KD |
| 观测分子量 | 25 kDa |
| GenBank蛋白编号 | NM_008491.1 |
| 基因名称 | NGAL |
| Gene ID (NCBI) | 16819 |
| RRID | AB_3670815 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P11672 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Neutrophil gelatinase-associated lipocalin (NGAL, also known as lipocalin-2, siderocalin, or LCN2) is a small molecule of almost 25 kDa that belongs to the well-defined superfamily of proteins called lipocalins. NGAL was initially found in activated neutrophils, under its role as an innate antibacterial factor. However, it subsequently was shown that many other types of cells, including in the kidney tubule, may produce NGAL in response to various injuries. NGAL may become one of the most promising next-generation biomarkers in clinical nephrology and beyond. This antibody recognizes mouse Lipocalin-2/NGAL.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Lipocalin-2/NGAL antibody 83102-1-RR | Download protocol |
| IHC protocol for Lipocalin-2/NGAL antibody 83102-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
