Lipocalin-2/Ngal Recombinant antibody

Lipocalin-2/Ngal Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

230372D6

Cat no : 83102-1-RR

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Synonyms

24p3, AW212229, Lcn2, lipocalin 2, NGAL



经过测试的应用

Positive WB detected inmouse lung tissue, mouse uterus tissue
Positive IHC detected inmouse spleen tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83102-1-RR targets Lipocalin-2/Ngal in WB, IHC, ELISA applications and shows reactivity with mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity mouse
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name lipocalin 2
Synonyms 24p3, AW212229, Lcn2, lipocalin 2, NGAL
Calculated Molecular Weight23KD
Observed Molecular Weight 25 kDa
GenBank Accession NumberNM_008491.1
Gene Symbol NGAL
Gene ID (NCBI) 16819
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11672
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neutrophil gelatinase-associated lipocalin (NGAL, also known as lipocalin-2, siderocalin, or LCN2) is a small molecule of almost 25 kDa that belongs to the well-defined superfamily of proteins called lipocalins. NGAL was initially found in activated neutrophils, under its role as an innate antibacterial factor. However, it subsequently was shown that many other types of cells, including in the kidney tubule, may produce NGAL in response to various injuries. NGAL may become one of the most promising next-generation biomarkers in clinical nephrology and beyond. This antibody recognizes mouse Ngal.

实验方案

Product Specific Protocols
WB protocol for Lipocalin-2/Ngal antibody 83102-1-RRDownload protocol
IHC protocol for Lipocalin-2/Ngal antibody 83102-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols