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Ninein Monoclonal antibody, PBS Only

Ninein Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Canine, mouse

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B10A8

Cat No : 67132-1-PBS

Print datasheet

Synonyms

GSK3B interacting protein, hNinein, KIAA1565, NIN, Ninein

验证数据展示

  • WB analysis using 67132-1-Ig (same clone as 67132-1-PBS)

    WB analysis using 67132-1-Ig (same clone as 67132-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67132-1-Ig (Ninein antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67132-1-PBS in a different storage buffer formulation.

  • IF Staining of MDCK using 67132-1-Ig (same clone as 67132-1-PBS)

    IF Staining of MDCK using 67132-1-Ig (same clone as 67132-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 67132-1-Ig (Ninein antibody) at dilution of 1:500 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67132-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67132-1-Ig (same clone as 67132-1-PBS)

    IHC staining of mouse brain using 67132-1-Ig (same clone as 67132-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67132-1-Ig (Ninein antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67132-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67132-1-Ig (same clone as 67132-1-PBS)

    IHC staining of mouse brain using 67132-1-Ig (same clone as 67132-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67132-1-Ig (Ninein antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67132-1-PBS in a different storage buffer formulation.

产品信息

67132-1-PBS targets Ninein in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Canine, mouse samples.

Tested Applications WB, IF, IHC, Indirect ELISA Application Description
Tested Reactivity Human, Canine, mouse
Immunogen Ninein fusion protein Ag14652 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ninein (GSK3B interacting protein)
Synonyms GSK3B interacting protein, hNinein, KIAA1565, NIN, Ninein
Calculated Molecular Weight 2090 aa, 243 kDa
Observed Molecular Weight 220 kDa
GenBank Accession NumberBC034708
Gene Symbol NIN
Gene ID (NCBI) 51199
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8N4C6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Ninein is a large coiled-coil protein that associates with the subdistal appendages of the mother centriole and the minus-ends of both centrioles. Ninein interacts with γ-tubulin and microtubule nucleation and anchoring at the centrosomes.