• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of HeLa using 84937-1-RR

    WB analysis of HeLa using 84937-1-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 84937-1-RR (Ninein antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84937-1-RR

    Affinity and Kinetic Characterization of 84937-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84937-1-RR against Human Ninein were performed. The affinity constant is 0.172 nM.

Ninein Recombinant antibody

Ninein Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84937-1-RR

Print datasheet

CloneNo. 242525B7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

NIN, KIAA1565, hNinein, GSK3B-interacting protein, GSK3B interacting protein

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84937-1-RR targets Ninein in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Ninein fusion protein Ag3688 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ninein (GSK3B interacting protein)
Synonyms NIN, KIAA1565, hNinein, GSK3B-interacting protein, GSK3B interacting protein
Calculated Molecular Weight 2090 aa, 243 kDa
Observed Molecular Weight 240-255 kDa
GenBank Accession NumberBC034708
Gene Symbol Ninein
Gene ID (NCBI) 51199
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8N4C6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ninein is a large coiled-coil protein that associates with the subdistal appendages of the mother centriole and the minus-ends of both centrioles. Ninein interacts with γ-tubulin and microtubule nucleation and anchoring at the centrosomes.

实验方案

Product Specific Protocols
WB protocol for Ninein antibody 84937-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols