ChromoTek Nuclear Actin-Chromobody® plasmid (TagGFP)
DNA plasmid encoding for anti-Actin VHH (anti-actin Nanobody)with nuclear localization signal (NLS) fused to TagGFP2.
Specificity
Actin
Applications
IF, Live cell imaging
Vector type
Mammalian expression vector
Reporter
TagGFP2
Cat No : acg-n
Synonyms
Validation Data Gallery
Product Information
DNA plasmid encoding for anti-Actin VHH (anti-actin Nanobody)with nuclear localization signal (NLS) fused to TagGFP2.
| Description | The nuclear Actin-Chromobody allows imaging of actin dynamics in real-time in living cells.
• Easily transfect your cells and non-invasively visualize actin cytoskeleton in real-time • Target and trace microfilaments in your cell line of interest • Use Actin-Chromobody as a marker of cellular morphology and as a probe for cellular motility • Actin-Chromobody has no notable effect cell viability or migration The nuclear Actin-Chromobody® plasmid has been published and described by Prof. Dr. Robert Grosse, Institute of Pharmacology, BPC, University of Marburg, Germany under license from ChromoTek:Matthias Plessner, Michael Melak, Pilar Chinchilla, Christian Baarlink, Robert Grosse (2015). Nuclear F-actin formation and reorganization upon cell spreading J Biol Chem. (Pubmed) |
| Applications | Live cell imaging Visualizing actin dynamics Actin as biomarker or control |
| Specificity | Actin microfilaments in human, rodent and plant cells, zebrafish, Toxoplasma gondii |
| Reporter | TagGFP2 |
| Vector type | Mammalian expression vector |
| Promoter | Constitutive CMV IE |
| Codon usage | Mammalian |
| Selection | Kan/Neo |
| Nuclear localization signal (NLS) | Yes |
| Sequence | With the Actin-Chromobody plasmid you obtain the sequence of the Alpaca antibody to Actin fused to TagRFP or TagGFP2, as well as the full vector sequence. |
| Transfection | Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type. |
| Microscopy techniques | Wide-field epifluorescence microscopy; confocal microscopy; super-resolution microscopy e.g. STED |
| Storage Condition | Shipped at ambient temperature. Store at -20 °C |
Documentation
| SDS |
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| SDS Nuclear Actin-Chromobody® TagGFP plasmid (PDF) |
| Datasheet |
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| Datasheet Nuclear Actin-Chromobody® TagGFP plasmid (PDF) |
| Brochure |
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| Chromotek nanobodies brochure (PDF) |
Publications
| Application | Title |
|---|---|
Nat Cell Biol Nuclear F-actin counteracts nuclear deformation and promotes fork repair during replication stress. | |
Nat Struct Mol Biol The nuclear actin-containing Arp8 module is a linker DNA sensor driving INO80 chromatin remodeling. | |
Mol Cell DNA damage induces nuclear envelope rupture through ATR-mediated phosphorylation of lamin A/C | |
Nat Commun Wiskott-Aldrich syndrome protein forms nuclear condensates and regulates alternative splicing. | |