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NUDCD3 Polyclonal antibody

NUDCD3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11764-1-AP

Print datasheet

Synonyms

NudCL, NudC domain-containing protein 3, KIAA1068

验证数据展示

  • IF Staining of HeLa using 11764-1-AP

    IF Staining of HeLa using 11764-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 11764-1-AP (NUDCD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis of human liver using 11764-1-AP

    WB analysis of human liver using 11764-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 11764-1-AP (NUDCD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 11764-1-AP

    IHC staining of human brain using 11764-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 11764-1-AP (NUDCD3 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 11764-1-AP

    IHC staining of human brain using 11764-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 11764-1-AP (NUDCD3 antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of mouse brain using 11764-1-AP

    IP experiment of mouse brain using 11764-1-AP

    IP result of anti-NUDCD3 (IP:11764-1-AP, 4ug; Detection:11764-1-AP 1:800) with mouse brain tissue lysate 4000ug.

经过测试的应用

Positive WB detected inhuman liver tissue, HEK-293 cells, HeLa cells, human brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

11764-1-AP targets NUDCD3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen NUDCD3 fusion protein Ag2362 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NudC domain containing 3
Synonyms NudCL, NudC domain-containing protein 3, KIAA1068
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC003691
Gene Symbol NUDCD3
Gene ID (NCBI) 23386
RRIDAB_2298665
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IVD9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NUDCD3 antibody 11764-1-APDownload protocol
IHC protocol for NUDCD3 antibody 11764-1-APDownload protocol
IF protocol for NUDCD3 antibody 11764-1-APDownload protocol
IP protocol for NUDCD3 antibody 11764-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Cell Dev Biol

NudC L279P Mutation Destabilizes Filamin A by Inhibiting the Hsp90 Chaperoning Pathway and Suppresses Cell Migration.

Authors - Min Liu
View Article
humanWB

Cancer Lett

Oncogenic functions and therapeutic potentials of targeted inhibition of SMARCAL1 in small cell lung cancer

Authors - Bei-Bei Sun
View Article