验证数据展示
经过测试的应用
Positive WB detected in | human liver tissue, HEK-293 cells, HeLa cells, human brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
产品信息
11764-1-AP targets NUDCD3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human, mouse |
文献引用反应性 | human |
免疫原 | NUDCD3 fusion protein Ag2362 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | NudC domain containing 3 |
别名 | NudCL, NudC domain-containing protein 3, KIAA1068 |
计算分子量 | 41 kDa |
观测分子量 | 50 kDa |
GenBank蛋白编号 | BC003691 |
基因名称 | NUDCD3 |
Gene ID (NCBI) | 23386 |
RRID | AB_2298665 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q8IVD9 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for NUDCD3 antibody 11764-1-AP | Download protocol |
IHC protocol for NUDCD3 antibody 11764-1-AP | Download protocol |
IF protocol for NUDCD3 antibody 11764-1-AP | Download protocol |
IP protocol for NUDCD3 antibody 11764-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Front Cell Dev Biol NudC L279P Mutation Destabilizes Filamin A by Inhibiting the Hsp90 Chaperoning Pathway and Suppresses Cell Migration. | ||
Cancer Lett Oncogenic functions and therapeutic potentials of targeted inhibition of SMARCAL1 in small cell lung cancer |