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验证数据展示

  • WB analysis using 65292-1-Ig

    WB analysis using 65292-1-Ig

    Untreated or PUGNAc (an inhibitor of N-acetyl-β-D-glucosaminidase) treated HeLa cells were subjected to SDS PAGE followed by western blot with 65292-1-Ig (O-GlcNAc antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

O-GlcNAc Monoclonal antibody

O-GlcNAc Monoclonal Antibody for WB
Cat No. 65292-1-Ig

产品说明书

CloneNo. CTD110.6

宿主/亚型

Mouse / IgM

种属反应性

n/a

应用

WB

别名

O-glycosylation, O-Glc, O Glc, glycosylation, O-linked β-N-acetylgulcosamine

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells

For this antibody, it is recommended to use BSA as the blocking agent, avoiding using milk in the blocking or dilution buffers.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65292-1-Ig targets O-GlcNAc in WB applications and shows reactivity with n/a samples.

经测试应用 WB Application Description
经测试反应性 n/a
免疫原 N/A 种属同源性预测
宿主/亚型 Mouse / IgM
抗体类别 Monoclonal
产品类型 Antibody
全称 O-GlcNAc
别名 O-glycosylation, O-Glc, O Glc, glycosylation, O-linked β-N-acetylgulcosamine
基因名称
Gene ID (NCBI)
RRIDAB_3670285
偶联类型 Unconjugated
形式 Liquid
纯化方式Affinity purification
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2-8°C. Stable for one year after shipment.

背景介绍

O-GlcNAc (O-linked β-N-acetylgulcosamine) is a post-translational modification of serine and threonine residues in proteins. O-GlcNAc modification is abundant in all multicellular eukaryotes, exclusively found on nuclear and cytoplasmic proteins rather than membrane proteins and secretory proteins (PMID: 11269319; 10924527). O-GlcNAc is attached to the protein backbone by enzymatic addition of the N-acetylgulcosamine (GlcNAc) moiety of uridine 5′-diphospho (UDP)-GlcNAc to the hydroxyl oxygen of serines or threonines by the O-linked β-N-acetylgulcosamine transferase (OGT). O-GlcNAc glycosylated proteins can be reversiblely deglycosylated by β-D-N-acetylgulcosaminase (O-GlcNAcase) (PMID: 21526146). O-GlcNAc modification couples many biological processes and plays important roles in development, normal physiology and physiopathology (PMID: 32349769).

实验方案

Product Specific Protocols
WB protocol for O-GlcNAc antibody 65292-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols