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OAS1 Recombinant antibody, PBS Only

OAS1 Uni-rAbTM Recombinant Antibody for Indirect ELISA, IHC, WB

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human

Applications

Indirect ELISA, IHC, WB

Conjugate

Unconjugated

CloneNo.

2H4

Cat no : 82883-1-PBS

Print datasheet

Synonyms

EC:2.7.7.84, E18/E16, 2H4, 2'-5'-oligoadenylate synthase 1, 2-5A synthase 1

验证数据展示

  • WB analysis using 82883-1-RR (same clone as 82883-1-PBS)

    WB analysis using 82883-1-RR (same clone as 82883-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82883-1-RR (OAS1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82883-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 82883-1-RR (same clone as 82883-1-PBS)

    IHC staining of mouse liver using 82883-1-RR (same clone as 82883-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82883-1-RR (OAS1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82883-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 82883-1-RR (same clone as 82883-1-PBS)

    FC experiment of HeLa using 82883-1-RR (same clone as 82883-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug OAS1 Recombinant antibody (82883-1-RR, Clone:2H4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82883-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82883-1-RR

    Affinity and Kinetic Characterization of 82883-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82883-1-RR against Human OAS1 were performed. The affinity constant is below 1 pM.

产品信息

82883-1-PBS targets OAS1 in Indirect ELISA, IHC, WB applications and shows reactivity with mouse, human samples.

Tested Applications Indirect ELISA, IHC, WB Application Description
Tested Reactivity mouse, human
Immunogen OAS1 fusion protein Ag6793 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name 2',5'-oligoadenylate synthetase 1, 40/46kDa
Synonyms EC:2.7.7.84, E18/E16, 2H4, 2'-5'-oligoadenylate synthase 1, 2-5A synthase 1
Calculated Molecular Weight 46 kDa
Observed Molecular Weight38 kDa
GenBank Accession NumberBC000562
Gene Symbol OAS1
Gene ID (NCBI) 4938
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP00973
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The 2-prime,5-prime oligoadenylate synthetases (OASs), such as OAS1, are interferon-induced proteins characterized by their capacity to catalyze the synthesis of 2-prime,5-prime oligomers of adenosine (2-5As). OAS1 (type I enzymes) has some isoforms with the MW of 40, 42, 44, 46, and 48 kDa (PMID:12590567, 19383565). OAS1 is a strong candidate for determining susceptibility or resistance to viral infections(PMID:15732009).