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验证数据展示

  • WB analysis using 82883-1-RR (same clone as 82883-1-PBS)

    WB analysis using 82883-1-RR (same clone as 82883-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82883-1-RR (OAS1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82883-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 82883-1-RR (same clone as 82883-1-PBS)

    IHC staining of mouse liver using 82883-1-RR (same clone as 82883-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82883-1-RR (OAS1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82883-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 82883-1-RR (same clone as 82883-1-PBS)

    FC experiment of HeLa using 82883-1-RR (same clone as 82883-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug OAS1 Recombinant antibody (82883-1-RR, Clone:2H4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82883-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82883-1-RR

    Affinity and Kinetic Characterization of 82883-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82883-1-RR against Human OAS1 were performed. The affinity constant is below 1 pM.

OAS1 Recombinant antibody, PBS Only

OAS1 Uni-rAbTM Recombinant Antibody for Indirect ELISA, IHC, WB
Cat No. 82883-1-PBS

产品说明书

CloneNo. 2H4

宿主/亚型

Rabbit / IgG

种属反应性

mouse, human

应用

Indirect ELISA, IHC, WB

别名

EC:2.7.7.84, E18/E16, 2H4, 2'-5'-oligoadenylate synthase 1, 2-5A synthase 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

82883-1-PBS targets OAS1 in Indirect ELISA, IHC, WB applications and shows reactivity with mouse, human samples.

经测试应用 Indirect ELISA, IHC, WB Application Description
经测试反应性 mouse, human
免疫原 OAS1 fusion protein Ag6793 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 2',5'-oligoadenylate synthetase 1, 40/46kDa
别名 EC:2.7.7.84, E18/E16, 2H4, 2'-5'-oligoadenylate synthase 1, 2-5A synthase 1
计算分子量 46 kDa
观测分子量38 kDa
GenBank蛋白编号BC000562
基因名称 OAS1
Gene ID (NCBI) 4938
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP00973
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The 2-prime,5-prime oligoadenylate synthetases (OASs), such as OAS1, are interferon-induced proteins characterized by their capacity to catalyze the synthesis of 2-prime,5-prime oligomers of adenosine (2-5As). OAS1 (type I enzymes) has some isoforms with the MW of 40, 42, 44, 46, and 48 kDa (PMID:12590567, 19383565). OAS1 is a strong candidate for determining susceptibility or resistance to viral infections(PMID:15732009).