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OAT Polyclonal antibody

OAT Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17089-1-AP

Print datasheet

Synonyms

Ornithine aminotransferase, hepatic form, OKT, OATASE, HOGA, GACR

验证数据展示

  • IHC staining of human liver cancer using 17089-1-AP

    IHC staining of human liver cancer using 17089-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17089-1-AP (OAT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 17089-1-AP

    IHC staining of human liver cancer using 17089-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17089-1-AP (OAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 17089-1-AP

    WB analysis using 17089-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17089-1-AP (OAT antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 17089-1-AP

    IF Staining of HeLa using 17089-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OAT antibody (17089-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inK-562 cells, mouse colon tissue, mouse lung tissue, rat colon tissue, rat lung tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17089-1-AP targets OAT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen OAT fusion protein Ag9981 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ornithine aminotransferase (gyrate atrophy)
Synonyms Ornithine aminotransferase, hepatic form, OKT, OATASE, HOGA, GACR
Calculated Molecular Weight 62 kDa
Observed Molecular Weight48 kDa
GenBank Accession NumberBC000964
Gene Symbol OAT
Gene ID (NCBI) 4942
RRIDAB_2878347
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04181
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for OAT antibody 17089-1-APDownload protocol
IHC protocol for OAT antibody 17089-1-APDownload protocol
IF protocol for OAT antibody 17089-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols