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验证数据展示

  • IHC staining of human liver cancer using 17089-1-AP

    IHC staining of human liver cancer using 17089-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17089-1-AP (OAT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 17089-1-AP

    IHC staining of human liver cancer using 17089-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17089-1-AP (OAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 17089-1-AP

    WB analysis using 17089-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17089-1-AP (OAT antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 17089-1-AP

    IF Staining of HeLa using 17089-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OAT antibody (17089-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

OAT Polyclonal antibody

OAT Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 17089-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Ornithine aminotransferase, hepatic form, OKT, OATASE, HOGA, GACR

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, mouse colon tissue, mouse lung tissue, rat colon tissue, rat lung tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17089-1-AP targets OAT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 OAT fusion protein Ag9981 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ornithine aminotransferase (gyrate atrophy)
别名 Ornithine aminotransferase, hepatic form, OKT, OATASE, HOGA, GACR
计算分子量48 kDa
观测分子量48 kDa
GenBank蛋白编号BC000964
基因名称 OAT
Gene ID (NCBI) 4942
RRIDAB_2878347
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP04181
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for OAT antibody 17089-1-APDownload protocol
IHC protocol for OAT antibody 17089-1-APDownload protocol
IF protocol for OAT antibody 17089-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols