• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

OATL1 Polyclonal antibody

OATL1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28597-1-AP

Print datasheet

Synonyms

TBC1D25, TBC1 domain family, member 25, TBC1 domain family member 25

验证数据展示

  • WB analysis using 28597-1-AP

    WB analysis using 28597-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28597-1-AP (OATL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, U2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28597-1-AP targets OATL1 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen OATL1 fusion protein Ag29470 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TBC1 domain family, member 25
Synonyms TBC1D25, TBC1 domain family, member 25, TBC1 domain family member 25
Observed Molecular Weight80-90 kDa
GenBank Accession NumberBC101817
Gene Symbol TBC1D25
Gene ID (NCBI) 4943
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ3MII6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OATL1, also known as TBC1D25, is a protein with a TBC domain and functions as a Rab GTPase activating protein. It is involved in the fusion of autophagosomes with endosomes and lysosomes. OATL1 is recruited to isolation membranes and autophagosomes through direct interaction with Atg8 homologues and is involved in the fusion between autophagosomes and lysosomes through its GAP activity.

实验方案

Product Specific Protocols
WB protocol for OATL1 antibody 28597-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols