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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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验证数据展示

  • WB analysis using 28597-1-AP

    WB analysis using 28597-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28597-1-AP (OATL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

OATL1 Polyclonal antibody

OATL1 Polyclonal Antibody for WB, ELISA
Cat No. 28597-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

TBC1D25, TBC1 domain family, member 25, TBC1 domain family member 25

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, U2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28597-1-AP targets OATL1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 OATL1 fusion protein Ag29470 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TBC1 domain family, member 25
别名 TBC1D25, TBC1 domain family, member 25, TBC1 domain family member 25
观测分子量80-90 kDa
GenBank蛋白编号BC101817
基因名称 TBC1D25
Gene ID (NCBI) 4943
RRIDAB_3669665
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ3MII6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OATL1, also known as TBC1D25, is a protein with a TBC domain and functions as a Rab GTPase activating protein. It is involved in the fusion of autophagosomes with endosomes and lysosomes. OATL1 is recruited to isolation membranes and autophagosomes through direct interaction with Atg8 homologues and is involved in the fusion between autophagosomes and lysosomes through its GAP activity.

实验方案

Product Specific Protocols
WB protocol for OATL1 antibody 28597-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols