- Featured Product
- KD/KO Validated
OBFC2B Polyclonal antibody
OBFC2B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, NIH/3T3 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human ovary tumor tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
产品信息
14809-1-AP targets OBFC2B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | OBFC2B fusion protein Ag6532 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | oligonucleotide/oligosaccharide-binding fold containing 2B |
| Synonyms | Sensor of single-strand DNA complex subunit B1, Oligonucleotide/oligosaccharide-binding fold-containing protein 2B, Nucleic acid-binding protein 2, NABP2, LP3587 |
| Calculated Molecular Weight | 22 kDa |
| Observed Molecular Weight | 30-33 kDa |
| GenBank Accession Number | BC001079 |
| Gene Symbol | OBFC2B |
| Gene ID (NCBI) | 79035 |
| RRID | AB_2251673 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BQ15 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
OBFC2B, also named as NABP2 or SSB1, is a 211 amino acid protein, which belongs to the SOSS-B family. OBFC2B localizes to nuclear foci following DNA damage. OBFC2B is a component of the SOSS complex, a multiprotein complex that functions downstream of the MRN complex to promote DNA repair and G2/M checkpoint. In the SOSS complex, OBFC2B acts as a sensor of single-stranded DNA that binds to single-stranded DNA, in particular to polypyrimidines. The calculated molecular weight of OBFC2B is 22 kDa, but modified OBFC2B is about 32 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for OBFC2B antibody 14809-1-AP | Download protocol |
| IHC protocol for OBFC2B antibody 14809-1-AP | Download protocol |
| IF protocol for OBFC2B antibody 14809-1-AP | Download protocol |
| IP protocol for OBFC2B antibody 14809-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Res The Integrator complex controls the termination of transcription at diverse classes of gene targets.
| ||
Cell Death Dis Nitric oxide triggers the assembly of "type II" stress granules linked to decreased cell viability. | ||
J Inflamm Res Comprehensive Analysis of NABP2 as a Prognostic Biomarker and Its Correlation with Immune Infiltration in Hepatocellular Carcinoma |
