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验证数据展示

  • WB analysis using 16634-1-AP

    WB analysis using 16634-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16634-1-AP (OCIAD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 16634-1-AP

    IHC staining of human liver cancer using 16634-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16634-1-AP (OCIAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 16634-1-AP

    IHC staining of human liver cancer using 16634-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16634-1-AP (OCIAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 16634-1-AP

    IF Staining of HepG2 using 16634-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using OCIAD1 antibody (16634-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • IP experiment of HeLa using 16634-1-AP

    IP experiment of HeLa using 16634-1-AP

    IP result of anti-OCIAD1 (IP:16634-1-AP, 4ug; Detection:16634-1-AP 1:300) with HeLa cells lysate 3200ug.

OCIAD1 Polyclonal antibody

OCIAD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 16634-1-AP

Print datasheet

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Synonyms

TPA018, Ovarian cancer immunoreactive antigen domain containing 1, OCIA domain-containing protein 1, OCIA domain containing 1, OCIA

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, human placenta tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue, human kidney tissue, human pancreas cancer tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16634-1-AP targets OCIAD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen OCIAD1 fusion protein Ag9977 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name OCIA domain containing 1
Synonyms TPA018, Ovarian cancer immunoreactive antigen domain containing 1, OCIA domain-containing protein 1, OCIA domain containing 1, OCIA
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 29-35 kDa
GenBank Accession NumberBC003409
Gene Symbol OCIAD1
Gene ID (NCBI) 54940
RRIDAB_2156297
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NX40
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OCIAD1 was first identified by immunoscreening of an ovarian carcinoma cDNA expression library with ascites fluid from ovarian cancer patients (PMID: 11162530). OCIAD1 has been reported as a key player in ovarian cancer cell adhesion, as well as a key player in generating ovarian cancer recurrence (PMID: 18328549; 20515946). In addition to its roles in cancer, OCIAD1 participates in maintaining stem cell potency by regulating the Jak/STAT pathway (PMID: 23972987). Several alternatively spliced forms of OCIAD1 gene have been identified. The longest form (1.4 kb) is predicted to encode for a 27.6 kDa protein of 245 amino acids. This antibody detects OCIAD1 with an apparent molecular weight of ~35 kDa as has been demonstrated by several researches (PMID: 27345969; 27345976).

实验方案

Product Specific Protocols
WB protocol for OCIAD1 antibody 16634-1-APDownload protocol
IHC protocol for OCIAD1 antibody 16634-1-APDownload protocol
IF protocol for OCIAD1 antibody 16634-1-APDownload protocol
IP protocol for OCIAD1 antibody 16634-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

PLoS One

OCIAD1 is a host mitochondrial substrate of the hepatitis C virus NS3-4A protease.

Authors - Huong T L Tran
View Article
humanIHC

Pathol Int

Increased expression of OCIA domain containing 2 during stepwise progression of ovarian mucinous tumor.

Authors - Nagata Chigusa C
View Article