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验证数据展示

  • WB analysis of HeLa using 66698-1-Ig (same clone as 66698-1-PBS)

    WB analysis of HeLa using 66698-1-Ig (same clone as 66698-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66698-1-Ig (OCIAD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66698-1-Ig (same clone as 66698-1-PBS)

    IHC staining of human liver cancer using 66698-1-Ig (same clone as 66698-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66698-1-Ig (OCIAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with OCIAD1 antibody included
  • IHC staining of human liver cancer using 66698-1-Ig (same clone as 66698-1-PBS)

    IHC staining of human liver cancer using 66698-1-Ig (same clone as 66698-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66698-1-Ig (OCIAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with OCIAD1 antibody included
  • IF Staining of human liver cancer using 66698-1-Ig (same clone as 66698-1-PBS)

    IF Staining of human liver cancer using 66698-1-Ig (same clone as 66698-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using OCIAD1 antibody (66698-1-Ig, Clone: 1C10C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 66698-1-Ig (same clone as 66698-1-PBS)

    IF Staining of human liver cancer using 66698-1-Ig (same clone as 66698-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using OCIAD1 antibody (66698-1-Ig, Clone: 1C10C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66698-1-Ig (same clone as 66698-1-PBS)

    IF Staining of HeLa using 66698-1-Ig (same clone as 66698-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OCIAD1 antibody (66698-1-Ig, Clone: 1C10C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50614-1

    Cytometric bead array standard curve of MP50614-1

    Cytometric bead array standard curve of MP50614-1, OCIAD1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66698-1-PBS. Detection antibody: 66698-2-PBS. Standard:Ag9977. Range: 0.098-100 ng/mL.

  • Cytometric bead array standard curve of MP50614-2

    Cytometric bead array standard curve of MP50614-2

    Cytometric bead array standard curve of MP50614-2, OCIAD1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66698-1-PBS. Detection antibody: 66698-3-PBS. Standard:Ag9977. Range: 0.098-100 ng/mL.

  • Featured Product
  • KD/KO验证

OCIAD1 Monoclonal antibody, PBS Only (Capture)

OCIAD1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 66698-1-PBS

产品说明书

CloneNo. 1C10C3

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

别名

Ovarian cancer immunoreactive antigen domain containing 1, OCIA domain-containing protein 1, OCIA domain containing 1, OCIA, ASRIJ

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® OCIAD1 Ready-To-Use IHC Kit. OCIAD1 primary antibody included.

产品信息

66698-1-PBS targets OCIAD1 as part of a matched antibody pair:

MP50614-1: 66698-1-PBS capture and 66698-2-PBS detection (validated in Cytometric bead array)

MP50614-2: 66698-1-PBS capture and 66698-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9977

Product name: Recombinant human OCIAD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-245 aa of BC003409

Sequence: MNGRADFREPNAEVPRPIPHIGPDYIPTEEERRVFAECNDESFWFRSVPLAATSMLITQGLISKGILSSHPKYGSIPKLILACIMGYFAGKLSYVKTCQEKFKKLENSPLGEALRSGQARRSSPPGHYYQKSKYDSSVSGQSSFVTSPAADNIEMLPHYEPIPFSSSMNESAPTGITDHIVQGPDPNLEESPKRKNITYEELRNKNRESYEVSLTQKTDPSVRPMHERVPKKEVKVNKYGDTWDE

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 OCIA domain containing 1
别名 Ovarian cancer immunoreactive antigen domain containing 1, OCIA domain-containing protein 1, OCIA domain containing 1, OCIA, ASRIJ
计算分子量 28 kDa
观测分子量 35 kDa
GenBank蛋白编号BC003409
基因名称 OCIAD1
Gene ID (NCBI) 54940
RRIDAB_2882051
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NX40
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

OCIAD1 was first identified by immunoscreening of an ovarian carcinoma cDNA expression library with ascites fluid from ovarian cancer patients (PMID: 11162530). OCIAD1 has been reported as a key player in ovarian cancer cell adhesion, as well as a key player in generating ovarian cancer recurrence (PMID: 18328549; 20515946). In addition to its roles in cancer, OCIAD1 participates in maintaining stem cell potency by regulating the Jak/STAT pathway (PMID: 23972987). Several alternatively spliced forms of OCIAD1 gene have been identified. The longest form (1.4 kb) is predicted to encode for a 27.6 kDa protein of 245 amino acids. This antibody detects OCIAD1 with an apparent molecular weight of ~35 kDa as has been demonstrated by several researches (PMID: 27345969; 27345976).

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