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验证数据展示

  • WB analysis of RAW264.7 using 23454-1-AP

    WB analysis of RAW264.7 using 23454-1-AP

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 23454-1-AP (ODC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate hyperplasia using 23454-1-AP

    IHC staining of human prostate hyperplasia using 23454-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 23454-1-AP (ODC1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human prostate hyperplasia using 23454-1-AP

    IHC staining of human prostate hyperplasia using 23454-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 23454-1-AP (ODC1 antibody) at dilution of 1:50 (under 40x lens).

ODC1 Polyclonal antibody

ODC1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 23454-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

ODC, ODC1, Ornithine decarboxylase, ornithine decarboxylase 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inRAW264.7 cells
Positive IHC detected inhuman prostate hyperplasia tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23454-1-AP targets ODC1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen ODC1 fusion protein Ag10927 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ornithine decarboxylase 1
Synonyms ODC, ODC1, Ornithine decarboxylase, ornithine decarboxylase 1
Calculated Molecular Weight 461 aa, 51 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC025296
Gene Symbol ODC1
Gene ID (NCBI) 4953
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP11926
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ornithine decarboxylase (ODC) is also named as ODC1 and belongs to the Orn/Lys/Arg decarboxylase class-II family. It catalyzes the conversion of ornithine to putrescine, the first step and a major site of regulation of polyamine biosynthesis. The level of ODC is known to be controlled at several sites, namely transcription, translation, and enzyme degradation. Polyamines can stimulate the degradation of ODC as a type of negative feedback control (PMID:8486633). This protein can be phosphorylated in vivo (PMID:8798774). ODC1 can form a homodimer and only the dimer is catalytically active, as the active sites are constructed of residues from both monomers (PMID: 10623504). The molecular mass of ODC1 is 51 kDa, and the homodimer is 106 kDa.

实验方案

Product Specific Protocols
WB protocol for ODC1 antibody 23454-1-APDownload protocol
IHC protocol for ODC1 antibody 23454-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols