ODF1 Polyclonal antibody
ODF1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | rat testis tissue, mouse testis tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
The immunogen of 24736-1-AP is ODF1 Fusion Protein expressed in E. coli.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, rat, mouse |
| Cited Reactivity | human, mouse, pig |
| Immunogen | ODF1 fusion protein Ag20562 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | outer dense fiber of sperm tails 1 |
| Synonyms | HSPB10, ODF, ODF1, ODF2, ODF27, ODFP, ODFPG, ODFPGA, ODFPGB, Outer dense fiber protein 1, RT7, SODF |
| Calculated Molecular Weight | 250 aa, 28 kDa |
| Observed Molecular Weight | 28 kDa, 56 kDa |
| GenBank Accession Number | BC104457 |
| Gene Symbol | ODF1 |
| Gene ID (NCBI) | 4956 |
| RRID | AB_2879697 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14990 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ODF1, also named as Outer dense fiber protein 1, is a 250 amino acid protein, which contains the C-terminal contains many C-X-P repeats. ODF1 is a component of the outer dense fibers (ODF) of spermatozoa. ODF27 that suggested the possibility of inter- actions between ODF27 itself and/or between ODF27 and other sperm tail proteins.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ODF1 antibody 24736-1-AP | Download protocol |
| IHC protocol for ODF1 antibody 24736-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol Comparative Proteomics and Phosphoproteomics Analysis Reveal the Possible Breed Difference in Yorkshire and Duroc Boar Spermatozoa. | ||
Mol Genet Genomic Med Novel mutations of PMFBP1 in a man with acephalic spermatozoa defects. | ||
Cell Mol Life Sci CCDC146 is required for sperm flagellum biogenesis and male fertility in mice |
