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OGDH Monoclonal antibody, PBS Only

OGDH Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H7A2

Cat no : 66285-1-PBS

Print datasheet

Synonyms

AKGDH, E1k, OGDC, OGDC E1, OGDH

验证数据展示

  • WB analysis of human heart using 66285-1-Ig (same clone as 66285-1-PBS)

    WB analysis of human heart using 66285-1-Ig (same clone as 66285-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 66285-1-Ig (OGDH Antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66285-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66285-1-Ig (same clone as 66285-1-PBS)

    IHC staining of human lung cancer using 66285-1-Ig (same clone as 66285-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66285-1-Ig (OGDH Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66285-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66285-1-Ig (same clone as 66285-1-PBS)

    IHC staining of human lung cancer using 66285-1-Ig (same clone as 66285-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66285-1-Ig (OGDH Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66285-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66285-1-Ig (same clone as 66285-1-PBS)

    IF Staining of HepG2 using 66285-1-Ig (same clone as 66285-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using OGDH antibody (66285-1-Ig, Clone: 1H7A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66285-1-PBS in a different storage buffer formulation.

产品信息

66285-1-PBS targets OGDH in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, rat
Immunogen OGDH fusion protein Ag7484 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
Synonyms AKGDH, E1k, OGDC, OGDC E1, OGDH
Calculated Molecular Weight 116 kDa
Observed Molecular Weight 116 kDa
GenBank Accession NumberBC004964
Gene Symbol OGDH
Gene ID (NCBI) 4967
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ02218
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

OGDH(2-oxoglutarate dehydrogenase, mitochondrial) is also named as OGDC-E1and belongs to the alpha-ketoglutarate dehydrogenase family. It is one of the three enzymatic components of 2-oxoglutarate dehydrogenase complex,which catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. This protein has 3 isoforms produced by alternative splicing.