验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, DU 145 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30800-1-AP targets OGFOD1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | OGFOD1 fusion protein Ag34034 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | 2-oxoglutarate and iron-dependent oxygenase domain containing 1 |
| 别名 | TPA1, Prolyl 3-hydroxylase OGFOD1, OGFOD 1, KIAA1612, EC:1.14.11.- |
| 计算分子量 | 542 aa, 63 kDa |
| 观测分子量 | 75 kDa |
| GenBank蛋白编号 | BC032919 |
| 基因名称 | OGFOD1 |
| Gene ID (NCBI) | 55239 |
| RRID | AB_3086419 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N543 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
2-oxoglutarate and iron-dependent oxygenase domain-containing protein 1 (OGFOD1) is a proly hydroxylase which belongs to 2-OG-Fe (II) dioxygenase family, it also namely KIAA1612 and TPA1. OGFOD1 is expressed in many tissues, mainly located in the nucleoplasm. It increased in many cancers and regulates both transcription and translation. The apparent molecular weight is larger than the calculated molecular weight of 63 kDa, which is likely due to posttranslational modification, presumably by glycosylation. (PMID:34298635 and 20579638)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for OGFOD1 antibody 30800-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |