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OGFOD2 Polyclonal antibody

OGFOD2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21590-1-AP

Print datasheet

Synonyms

OGFOD2

验证数据展示

  • WB analysis using 21590-1-AP

    WB analysis using 21590-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21590-1-AP (OGFOD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 21590-1-AP

    IHC staining of mouse brain using 21590-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21590-1-AP (OGFOD2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, human testis tissue, human skeletal muscle tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inmouse brain tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21590-1-AP is OGFOD2 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen OGFOD2 fusion protein Ag16164 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name 2-oxoglutarate and iron-dependent oxygenase domain containing 2
Synonyms OGFOD2
Calculated Molecular Weight 350 aa, 39 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC098293
Gene Symbol OGFOD2
Gene ID (NCBI) 79676
RRIDAB_10734327
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6N063
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OGFOD2 belongs to the OGFOD2 family. OGFOD2 has 4 isoforms with the molecular mass of 15, 20, 32 and 39 kDa.

实验方案

Product Specific Protocols
WB protocol for OGFOD2 antibody 21590-1-APDownload protocol
IHC protocol for OGFOD2 antibody 21590-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols