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  • KD/KO Validated

OGFR Polyclonal antibody

OGFR Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 11177-1-AP

Print datasheet

Synonyms

Zeta-type opioid receptor, Zeta type opioid receptor, Protein 7-60, Protein 7 60, Opioid growth factor receptor



经过测试的应用

Positive WB detected inCOLO 320 cells, A549 cells, HEK-293 cells, L02 cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman colon cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCOLO 320 cells, L02 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11177-1-AP targets OGFR in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen OGFR fusion protein Ag1671 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name opioid growth factor receptor
Synonyms Zeta-type opioid receptor, Zeta type opioid receptor, Protein 7-60, Protein 7 60, Opioid growth factor receptor
Calculated Molecular Weight 73 kDa
Observed Molecular Weight 90-110 kDa
GenBank Accession NumberBC014137
Gene Symbol OGFR
Gene ID (NCBI) 11054
RRIDAB_2156772
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZT2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for OGFR antibody 11177-1-APDownload protocol
IHC protocol for OGFR antibody 11177-1-APDownload protocol
IF protocol for OGFR antibody 11177-1-APDownload protocol
IP protocol for OGFR antibody 11177-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Biol Sci

CIRBP-OGFR axis safeguards against cardiomyocyte apoptosis and cardiotoxicity induced by chemotherapy.

Authors - Cihang Liu
  • KD Validated
humanIHC

Front Oncol

Opioid Receptor Expression in Colorectal Cancer: A Nested Matched Case-Control Study.

Authors - Amparo Belltall
humanIHC

Int Immunopharmacol

Methionine enkephalin inhibited cervical carcinoma via apoptosis promotion and reduction of myeloid derived suppressor cell infiltrated in tumor.

Authors - Na Qu
humanWB, IHC

Int Immunopharmacol

Methionine enkephalin activates autophagy and stimulates tumour cell immunogenicity in human cutaneous squamous cell carcinoma.

Authors - Xueli Bai
humanWB, IHC, IF

Int Immunopharmacol

Regulatory role of methionine enkephalin in myeloid-derived suppressor cells and macrophages in human cutaneous squamous cell carcinoma.

Authors - Xueli Bai
humanWB,IHC

Int Immunopharmacol

A novel mechanism of lung cancer inhibition by methionine enkephalin through remodeling the immune status of the tumor microenvironment.

Authors - Shuling Zhang
  • KD Validated