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  • Featured Product
  • KD/KO Validated

OGT Monoclonal antibody, PBS Only

OGT Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B2A6

Cat No : 66823-1-PBS

Print datasheet

Synonyms

FLJ23071, HRNT1, O GLCNAC, OGT

验证数据展示

  • WB analysis using 66823-1-Ig (same clone as 66823-1-PBS)

    WB analysis using 66823-1-Ig (same clone as 66823-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66823-1-Ig (OGT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66823-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66823-1-Ig (same clone as 66823-1-PBS)

    IHC staining of human lung cancer using 66823-1-Ig (same clone as 66823-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66823-1-Ig (OGT antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66823-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with OGT antibody included
  • IHC staining of human lung cancer using 66823-1-Ig (same clone as 66823-1-PBS)

    IHC staining of human lung cancer using 66823-1-Ig (same clone as 66823-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66823-1-Ig (OGT antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66823-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with OGT antibody included
  • IF Staining of HepG2 using 66823-1-Ig (same clone as 66823-1-PBS)

    IF Staining of HepG2 using 66823-1-Ig (same clone as 66823-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using OGT antibody (66823-1-Ig, Clone: 2B2A6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66823-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy OGT Ready-To-Use IHC Kit. OGT primary antibody included.

产品信息

66823-1-PBS targets OGT in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications WB, IF, IHC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat, pig
Immunogen OGT fusion protein Ag28402 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
Synonyms FLJ23071, HRNT1, O GLCNAC, OGT
Calculated Molecular Weight 1046 aa, 117 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC014434
Gene Symbol OGT
Gene ID (NCBI) 8473
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15294
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

O-linked N-acetylglucosamine transferase (OGT) catalyzes the attachment of N-acetylglucosamine (GlcNAc) monosaccharides to the hydroxyl group of serine or threonine residues of numerous nuclear and cytoplasmic proteins and may play important roles in a large number of diverse intracellular processes ranging from translational control, transcription, transcriptional repression, INS resistance and regulation of the cell cycle. It exists as a heterotrimeric complex with two 110 kDa and one 70 kDa subunits. Recent studies have shown that O-GlcNAcylation plays essential roles in cancer formation and progression. O-GlcNAcylation as well as OGT expression was found to be significantly elevated in the cancer tissues.