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验证数据展示

  • WB analysis using 66823-1-Ig (same clone as 66823-1-PBS)

    WB analysis using 66823-1-Ig (same clone as 66823-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66823-1-Ig (OGT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66823-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66823-1-Ig (same clone as 66823-1-PBS)

    IHC staining of human lung cancer using 66823-1-Ig (same clone as 66823-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66823-1-Ig (OGT antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66823-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with OGT antibody included
  • IHC staining of human lung cancer using 66823-1-Ig (same clone as 66823-1-PBS)

    IHC staining of human lung cancer using 66823-1-Ig (same clone as 66823-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66823-1-Ig (OGT antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66823-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with OGT antibody included
  • IF Staining of HepG2 using 66823-1-Ig (same clone as 66823-1-PBS)

    IF Staining of HepG2 using 66823-1-Ig (same clone as 66823-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using OGT antibody (66823-1-Ig, Clone: 2B2A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66823-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

OGT Monoclonal antibody, PBS Only

OGT Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66823-1-PBS

产品说明书

CloneNo. 2B2A6

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat, pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

FLJ23071, HRNT1, O GLCNAC, OGT

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy OGT Ready-To-Use IHC Kit. OGT primary antibody included.

产品信息

66823-1-PBS targets OGT in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat, pig
免疫原 OGT fusion protein Ag28402 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
别名 FLJ23071, HRNT1, O GLCNAC, OGT
计算分子量 1046 aa, 117 kDa
观测分子量 110 kDa
GenBank蛋白编号BC014434
基因名称 OGT
Gene ID (NCBI) 8473
RRIDAB_2882166
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO15294
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

O-linked N-acetylglucosamine transferase (OGT) catalyzes the attachment of N-acetylglucosamine (GlcNAc) monosaccharides to the hydroxyl group of serine or threonine residues of numerous nuclear and cytoplasmic proteins and may play important roles in a large number of diverse intracellular processes ranging from translational control, transcription, transcriptional repression, INS resistance and regulation of the cell cycle. It exists as a heterotrimeric complex with two 110 kDa and one 70 kDa subunits. Recent studies have shown that O-GlcNAcylation plays essential roles in cancer formation and progression. O-GlcNAcylation as well as OGT expression was found to be significantly elevated in the cancer tissues.