验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, Jurkat cells |
| Positive IP detected in | Jurkat cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12142-1-AP targets OIP5 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | OIP5 fusion protein Ag2790 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | Opa interacting protein 5 |
| Synonyms | 5730547N13Rik, Cancer/testis antigen 86, CT86, LINT 25, MIS18B, MIS18beta, OIP 5, OIP5, Opa interacting protein 5, Protein Mis18 beta |
| Calculated Molecular Weight | 229 aa, 25 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | BC015050 |
| Gene Symbol | OIP5 |
| Gene ID (NCBI) | 11339 |
| RRID | AB_2157076 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43482 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
OIP5 is also known as Mis18β. Vertebrates possess two Mis18 paralogs, Mis18α and Mis18β. Mis18 has been previously shown to affect histone modifications and the methylation status of underlying chromatin . Eliminating histone methylation within the alpha satellite DNA repeats of a human artificial chromosome alters the recruitment of HJURP .The Mis18core complex was originally characterized as an oligomer with a proposed 2:2 stoichiometry of Mis18α and Mis18β, but additional work demonstrated a 4:2 Mis18α:Mis18β hexamer. (PMID: 31492860, PMID: 26942680)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for OIP5 antibody 12142-1-AP | Download protocol |
| IP protocol for OIP5 antibody 12142-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Bull (Beijing) Engineering redirected NF-κB/OIP5 expression programs to enhance tumor responses to chemotherapy in bladder cancer | ||
Neuro Oncol Cancer-testis specific gene OIP5: a downstream gene of E2F1 that promotes tumorigenesis and metastasis in glioblastoma by stabilizing E2F1 signaling.
| ||
Oncotarget OIP5, a target of miR-15b-5p, regulates hepatocellular carcinoma growth and metastasis through the AKT/mTORC1 and β-catenin signaling pathways. | ||
J Biol Chem Mitotic regulator Mis18β interacts with and specifies the centromeric assembly of molecular chaperone HJURP.
| ||
Int J Oncol Characterization of Opa interacting protein 5 as a new biomarker and therapeutic target for oral cancer.
| ||
Cancer Sci Characterization of an Opa interacting protein 5 involved in lung and esophageal carcinogenesis. |
