验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells, COLO 320 cells, Raji cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66461-1-Ig targets OIP5 in WB, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB, IP, CoIP |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | OIP5 fusion protein Ag2790 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | Opa interacting protein 5 |
| Synonyms | 5730547N13Rik, Cancer/testis antigen 86, CT86, LINT 25, MIS18B, MIS18beta, OIP 5, OIP5, Opa interacting protein 5, Protein Mis18 beta |
| Calculated Molecular Weight | 229 aa, 25 kDa |
| Observed Molecular Weight | 25-30 kDa |
| GenBank Accession Number | BC015050 |
| Gene Symbol | OIP5 |
| Gene ID (NCBI) | 11339 |
| RRID | AB_2881830 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O43482 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
OIP5, also named as IS18B and CT86, is required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis. It is over expressed in the majority of primary lung and esophageal cancers. OIP5 is a promising target for developing new prognostic biomarkers and anti-cancer drugs.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for OIP5 antibody 66461-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |