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  • Featured Product
  • KD/KO Validated

OIP5 Monoclonal antibody

OIP5 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, ELISA and More (2)

Conjugate

Unconjugated

CloneNo.

1E9B5

Cat no : 66461-1-Ig

Print datasheet

Synonyms

5730547N13Rik, Cancer/testis antigen 86, CT86, LINT 25, MIS18B, MIS18beta, OIP 5, OIP5, Opa interacting protein 5, Protein Mis18 beta

验证数据展示

  • WB analysis using 66461-1-Ig

    WB analysis using 66461-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66461-1-Ig (OIP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 66461-1-Ig

    WB analysis of Jurkat using 66461-1-Ig

    WB result of OIP5 antibody (66461-1-Ig; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-OIP5 transfected Jurkat cells.

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, COLO 320 cells, Raji cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66461-1-Ig targets OIP5 in WB, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB, IP, CoIP
Tested Reactivity human
Cited Reactivityhuman
Immunogen OIP5 fusion protein Ag2790 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name Opa interacting protein 5
Synonyms 5730547N13Rik, Cancer/testis antigen 86, CT86, LINT 25, MIS18B, MIS18beta, OIP 5, OIP5, Opa interacting protein 5, Protein Mis18 beta
Calculated Molecular Weight 229 aa, 25 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC015050
Gene Symbol OIP5
Gene ID (NCBI) 11339
RRIDAB_2881830
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43482
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OIP5, also named as IS18B and CT86, is required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis. It is over expressed in the majority of primary lung and esophageal cancers. OIP5 is a promising target for developing new prognostic biomarkers and anti-cancer drugs.

实验方案

Product Specific Protocols
WB protocol for OIP5 antibody 66461-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP,CoIP

Oncogene

Engineered targeting OIP5 sensitizes bladder cancer to chemotherapy resistance via TRIP12-PPP1CB-YBX1 axis

Authors - Xianteng Wang
  • KD Validated
View Article