验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells, COLO 320 cells, Raji cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66461-1-Ig targets OIP5 in WB, IP, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IP, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | OIP5 fusion protein Ag2790 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | Opa interacting protein 5 |
| 别名 | 5730547N13Rik, Cancer/testis antigen 86, CT86, LINT 25, MIS18B, MIS18beta, OIP 5, OIP5, Opa interacting protein 5, Protein Mis18 beta |
| 计算分子量 | 229 aa, 25 kDa |
| 观测分子量 | 25-30 kDa |
| GenBank蛋白编号 | BC015050 |
| 基因名称 | OIP5 |
| Gene ID (NCBI) | 11339 |
| RRID | AB_2881830 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O43482 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
OIP5, also named as IS18B and CT86, is required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis. It is over expressed in the majority of primary lung and esophageal cancers. OIP5 is a promising target for developing new prognostic biomarkers and anti-cancer drugs.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for OIP5 antibody 66461-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |