验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, PC-3 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy IST1 Ready-To-Use IHC Kit. IST1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
51002-1-AP targets hIST1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | hIST1 fusion protein Ag0721 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | KIAA0174 |
| 别名 | hIST1, IST1, IST1 homolog, KIAA0174, OLC1 |
| 计算分子量 | 40 kDa |
| 观测分子量 | 40 kDa |
| GenBank蛋白编号 | BC000430 |
| 基因名称 | hIST1 |
| Gene ID (NCBI) | 9798 |
| RRID | AB_2130516 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P53990 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human increased sodium tolerance-1 (hIST1), encoded by KIAA0174 gene, is a newly reported endosomal sorting complexes required for transport (ESCRT)-III family member. IST1 possesses two different types of microtubule-interacting and transport (MIT) -interacting motifs (MIM1 and MIM2), this is required for its interaction with calpain 7 which is important for their roles in the ESCRT system.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for hIST1 antibody 51002-1-AP | Download protocol |
| IHC protocol for hIST1 antibody 51002-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nature Spastin and ESCRT-III coordinate mitotic spindle disassembly and nuclear envelope sealing.
| ||
J Cell Biol An ESCRT-spastin interaction promotes fission of recycling tubules from the endosome.
| ||
Am J Hum Genet De Novo VPS4A Mutations Cause Multisystem Disease with Abnormal Neurodevelopment. | ||
J Cell Biol ALIX and ESCRT-I/II function as parallel ESCRT-III recruiters in cytokinetic abscission. | ||
Proc Natl Acad Sci U S A ESCRT-III binding protein MITD1 is involved in cytokinesis and has an unanticipated PLD fold that binds membranes. |
