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验证数据展示

  • IHC staining of human lung cancer using 10079-1-AP

    IHC staining of human lung cancer using 10079-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 10079-1-AP (OLFM1 antibody) at dilution of 1:50 (under 10x lens).

OLFM1 Polyclonal antibody

OLFM1 Polyclonal Antibody for IHC, ELISA
Cat No. 10079-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10079-1-AP targets OLFM1 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen OLFM1 fusion protein Ag0125 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name olfactomedin 1
Synonyms
Calculated Molecular Weight 20 kDa
GenBank Accession NumberBC000189
Gene Symbol OLFM1
Gene ID (NCBI) 10439
RRIDAB_2157220
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99784
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Olfactomedin 1 (Olfm1), also known as noelin and pancortin, is a secreted glycoprotein belonging to a family of olfactomedin domain-containing proteins. The gene of Olfm1 is located on chromosome 9q34.3. Four variant transcripts (AMY, BMY, AMZ, and BMZ) are produced by alternative splicing. Olfm1 may be involved in the regulation of neural crest cell production, neuronal differentiation and ischemic neuronal death in adult. This antibody is specific for isoform 3 and isoform 4 (AMY) of Olfm1 with native molecular weights of 54 kDa and 16 kDa respectively. AMY may have an essential role in nerve tissue.

实验方案

Product Specific Protocols
IHC protocol for OLFM1 antibody 10079-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols