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OLFM3 Polyclonal antibody

OLFM3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 12596-1-AP

Print datasheet

Synonyms

Olfactomedin 3, Olfactomedin-3, Noelin-3, NOELIN3, Noelin 3

验证数据展示

  • WB analysis of PC-3 using 12596-1-AP

    WB analysis of PC-3 using 12596-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12596-1-AP (OLFM3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 12596-1-AP

    IF Staining of HepG2 using 12596-1-AP

    Immunofluorescent analysis of HepG2 cells, using OLFM3 antibody 12596-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IHC staining of human liver cancer using 12596-1-AP

    IHC staining of human liver cancer using 12596-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12596-1-AP (OLFM3 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver cancer using 12596-1-AP

    IHC staining of human liver cancer using 12596-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12596-1-AP (OLFM3 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inPC-3 cells, A2780 cells, human liver tissue, mouse liver tissue, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12596-1-AP targets OLFM3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen OLFM3 fusion protein Ag3207 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name olfactomedin 3
Synonyms Olfactomedin 3, Olfactomedin-3, Noelin-3, NOELIN3, Noelin 3
Calculated Molecular Weight 458 aa, 53 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC022531
Gene Symbol OLFM3
Gene ID (NCBI) 118427
RRIDAB_2157228
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96PB7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for OLFM3 antibody 12596-1-APDownload protocol
IHC protocol for OLFM3 antibody 12596-1-APDownload protocol
IF protocol for OLFM3 antibody 12596-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Front Cell Dev Biol

Olfactomedin-3 Enhances Seizure Activity by Interacting With AMPA Receptors in Epilepsy Models.

Authors - Shirong Tang
View Article