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OLFM4 Polyclonal antibody

OLFM4 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 28432-1-AP

Print datasheet

Synonyms

Antiapoptotic protein GW112, bA209J19.1, GC1, GW112, hGC 1, hOLfD, KIAA4294, olfactomedin 4, OlfD, OLFM4, OLM4, UNQ362

验证数据展示

  • IHC staining of human small intestine using 28432-1-AP

    IHC staining of human small intestine using 28432-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 28432-1-AP (OLFM4 antibody) at dilution of 1:1000 (under 10x lens).

  • IHC staining of human small intestine using 28432-1-AP

    IHC staining of human small intestine using 28432-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 28432-1-AP (OLFM4 antibody) at dilution of 1:1000 (under 40x lens).

经过测试的应用

Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

28432-1-AP targets OLFM4 in IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity Human
Cited Reactivitymouse
Immunogen OLFM4 fusion protein Ag28914 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name olfactomedin 4
Synonyms Antiapoptotic protein GW112, bA209J19.1, GC1, GW112, hGC 1, hOLfD, KIAA4294, olfactomedin 4, OlfD, OLFM4, OLM4, UNQ362
Calculated Molecular Weight 57 kDa
GenBank Accession NumberNM_006418
Gene Symbol OLFM4
Gene ID (NCBI) 10562
RRIDAB_2918163
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UX06
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Olfactomedin-4 (OLFM4), also named as hGC-1 (human G-CSF-stimulated clone-1) or antiapoptotic protein GW112, is a secreted glycoprotein normally expressed in bone marrow, prostate, small intestine, stomach, colon and pancreas (PMID: 11867215; PMID: 22471589). OLFM4 has been found to be up-regulated in gastrointestinal cancer and inflammatory disease including inflammatory bowel disease and H. pylori infection (PMID: 20534456). OLFM4 is involved in cellular process such as apoptosis, cell adhesion and tumor growth (PMID: 22471589; 16566923; 15059901).

实验方案

Product Specific Protocols
IHC protocol for OLFM4 antibody 28432-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Cell Rep

An ADAR1 dsRBD3-PKR kinase domain interaction on dsRNA inhibits PKR activation

Authors - Ketty Sinigaglia
View Article