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验证数据展示

  • IHC staining of human small intestine using 28432-1-AP

    IHC staining of human small intestine using 28432-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 28432-1-AP (OLFM4 antibody) at dilution of 1:1000 (under 10x lens).

    Ready-to-use IHC kits available with OLFM4 antibody included
  • IHC staining of human small intestine using 28432-1-AP

    IHC staining of human small intestine using 28432-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 28432-1-AP (OLFM4 antibody) at dilution of 1:1000 (under 40x lens).

    Ready-to-use IHC kits available with OLFM4 antibody included

OLFM4 Polyclonal antibody

OLFM4 Polyclonal Antibody for IHC, ELISA
Cat No. 28432-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

Human and More (1)

应用

IHC, ELISA and More (1)

别名

Antiapoptotic protein GW112, bA209J19.1, GC1, GW112, hGC 1, hOLfD, KIAA4294, olfactomedin 4, OlfD, OLFM4, OLM4, UNQ362

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy OLFM4 Ready-To-Use IHC Kit. OLFM4 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

28432-1-AP targets OLFM4 in IHC, IF, ELISA applications and shows reactivity with Human samples.

经测试应用 IHC, ELISA Application Description
文献引用应用IF
经测试反应性 Human
文献引用反应性mouse
免疫原 OLFM4 fusion protein Ag28914 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 olfactomedin 4
别名 Antiapoptotic protein GW112, bA209J19.1, GC1, GW112, hGC 1, hOLfD, KIAA4294, olfactomedin 4, OlfD, OLFM4, OLM4, UNQ362
计算分子量 57 kDa
GenBank蛋白编号NM_006418
基因名称 OLFM4
Gene ID (NCBI) 10562
RRIDAB_2918163
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6UX06
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Olfactomedin-4 (OLFM4), also named as hGC-1 (human G-CSF-stimulated clone-1) or antiapoptotic protein GW112, is a secreted glycoprotein normally expressed in bone marrow, prostate, small intestine, stomach, colon and pancreas (PMID: 11867215; PMID: 22471589). OLFM4 has been found to be up-regulated in gastrointestinal cancer and inflammatory disease including inflammatory bowel disease and H. pylori infection (PMID: 20534456). OLFM4 is involved in cellular process such as apoptosis, cell adhesion and tumor growth (PMID: 22471589; 16566923; 15059901).

实验方案

Product Specific Protocols
IHC protocol for OLFM4 antibody 28432-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Cell Rep

An ADAR1 dsRBD3-PKR kinase domain interaction on dsRNA inhibits PKR activation

Authors - Ketty Sinigaglia
View Article