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验证数据展示

  • WB analysis using 15869-1-AP

    WB analysis using 15869-1-AP

    mouse placenta tissue were subjected to SDS PAGE followed by western blot with 15869-1-AP (OLFML3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 15869-1-AP

    IHC staining of mouse heart using 15869-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15869-1-AP (OLFML3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

OLFML3 Polyclonal antibody

OLFML3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15869-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

PSEC0244, PSEC0173, PSEC0035, Olfactomedin-like protein 3, hOLF44

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse placenta tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15869-1-AP targets OLFML3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 OLFML3 fusion protein Ag8539 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 olfactomedin-like 3
别名 PSEC0244, PSEC0173, PSEC0035, Olfactomedin-like protein 3, hOLF44
计算分子量 406 aa, 46 kDa
观测分子量46-50 kDa
GenBank蛋白编号BC009920
基因名称 OLFML3
Gene ID (NCBI) 56944
RRIDAB_3669221
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NRN5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for OLFML3 antibody 15869-1-APDownload protocol
IHC protocol for OLFML3 antibody 15869-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols