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OLFML3 Polyclonal antibody

OLFML3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15869-1-AP

Print datasheet

Synonyms

PSEC0244, PSEC0173, PSEC0035, Olfactomedin-like protein 3, hOLF44

验证数据展示

  • WB analysis using 15869-1-AP

    WB analysis using 15869-1-AP

    mouse placenta tissue were subjected to SDS PAGE followed by western blot with 15869-1-AP (OLFML3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 15869-1-AP

    IHC staining of mouse heart using 15869-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15869-1-AP (OLFML3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse placenta tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15869-1-AP targets OLFML3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen OLFML3 fusion protein Ag8539 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name olfactomedin-like 3
Synonyms PSEC0244, PSEC0173, PSEC0035, Olfactomedin-like protein 3, hOLF44
Calculated Molecular Weight 406 aa, 46 kDa
Observed Molecular Weight46-50 kDa
GenBank Accession NumberBC009920
Gene Symbol OLFML3
Gene ID (NCBI) 56944
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRN5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for OLFML3 antibody 15869-1-APDownload protocol
IHC protocol for OLFML3 antibody 15869-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols