• Featured Product
  • KD/KO Validated

OLR1/LOX1 Polyclonal antibody

OLR1/LOX1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 11837-1-AP

Print datasheet

Synonyms

OLR1, Lectin-like oxidized LDL receptor 1, hLOX-1, hLOX1, hLOX 1



经过测试的应用

Positive WB detected inbEnd.3 cells, human liver tissue
Positive IP detected inL02 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11837-1-AP targets OLR1/LOX1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, CoIP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen OLR1/LOX1 fusion protein Ag2437 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name oxidized low density lipoprotein (lectin-like) receptor 1
Synonyms OLR1, Lectin-like oxidized LDL receptor 1, hLOX-1, hLOX1, hLOX 1
Calculated Molecular Weight 273 aa, 31 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC022295
Gene Symbol OLR1
Gene ID (NCBI) 4973
RRIDAB_2299037
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP78380
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OLR1/LOX-1 acts as a receptor, in the form of homodimer, that mediates the recognition, internalization, and degradation of oxidatively modified low density lipoprotein (oxLDL) (PMID: 9052782, 15695803). ORL1 is predominantly expressed in endothelial cells and vascular-rich organs such as the placenta, lung, liver, and brain (PMID: 9828121). OLR1 is a protein containing 273 amino acids with a calculated molecular mass of 31 kDa but an apparent molecular mass of 50 kDa, which may result from the glycosylation of four potential N-linked glycosylation sites located at the C-terminal domain (PMID: 9052782).

实验方案

Product Specific Protocols
WB protocol for OLR1/LOX1 antibody 11837-1-APDownload protocol
IHC protocol for OLR1/LOX1 antibody 11837-1-APDownload protocol
IP protocol for OLR1/LOX1 antibody 11837-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Free Radic Biol Med

Up-regulation of thioredoxin system by puerarin inhibits lipid uptake in macrophages

Authors - Wenchao Li,
humanWB

Cancer Lett

Up-regulation of OLR1 expression by TBC1D3 through activation of TNFα/NF-κB pathway promotes the migration of human breast cancer cells.

Authors - Bei Wang
  • KD Validated
humanWB

Metabolism

Deacetylation of Caveolin-1 by Sirt6 induces autophagy and retards high glucose-stimulated LDL transcytosis and atherosclerosis formation.

Authors - Ying Zhao
humanWB

Sci Rep

Lectin-like oxidized low-density lipoprotein receptor-1 facilitates metastasis of gastric cancer through driving epithelial-mesenchymal transition and PI3K/Akt/GSK3β activation.

Authors - Can Li
  • KD Validated
mouseIHC

Int J Cancer

The oxidized-LDL/LOX-1 axis in tumor endothelial cells enhances metastasis by recruiting neutrophils and cancer cells.

Authors - Takuya Tsumita
humanWB

Mol Cancer Res

OLR1 Promotes Pancreatic Cancer Metastasis via Increased c-Myc Expression and Transcription of HMGA2.

Authors - Gang Yang
  • KD Validated