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验证数据展示

  • WB analysis using 32465-1-AP

    WB analysis using 32465-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32465-1-AP (OPA1 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 32465-1-AP

    IP experiment of mouse brain using 32465-1-AP

    IP result of anti-OPA1 (IP:32465-1-AP, 4ug; Detection:32465-1-AP 1:40000) with mouse brain tissue lysate 1840 ug.

OPA1 Polyclonal antibody

OPA1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 32465-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

OPA1 gene, OPA 1, EC:3.6.5.5, Dynamin-like GTPase OPA1, short form, Dynamin-like GTPase OPA1, mitochondrial

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32465-1-AP targets OPA1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 OPA1 fusion protein Ag38295 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 optic atrophy 1 (autosomal dominant)
别名 OPA1 gene, OPA 1, EC:3.6.5.5, Dynamin-like GTPase OPA1, short form, Dynamin-like GTPase OPA1, mitochondrial
计算分子量 960 aa, 112 kDa
观测分子量80-100 kDa
GenBank蛋白编号BC075805
基因名称 OPA1
Gene ID (NCBI) 4976
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO60313
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OPA1 is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. OPA1 localizes to the inner mitochondrial membrane and helps regulate mitochondrial stability and energy output. This protein also sequesters cytochrome c. OPA1 is associated with the inner membrane and protects cells from apoptosis by regulating inner membrane dynamics. Mutation of OPA1 causes the disease dominant optic atrophy, a degeneration of the retinal ganglion cells. OPA1 undergoes complex posttranscriptional regulation and posttranslational proteolysis. OPA1 is regulated by proteolytic cleavage, which degrades long OPA1 isoforms into short isoforms.

实验方案

Product Specific Protocols
WB protocol for OPA1 antibody 32465-1-APDownload protocol
IP protocol for OPA1 antibody 32465-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols