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Oligophrenin 1 Polyclonal antibody

Oligophrenin 1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17445-1-AP

Print datasheet

Synonyms

MRX60, oligophrenin 1, OPHN1, OPN1

验证数据展示

  • WB analysis using 17445-1-AP

    WB analysis using 17445-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17445-1-AP (Oligophrenin 1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 17445-1-AP

    IP experiment of mouse brain using 17445-1-AP

    IP result of anti-Oligophrenin 1 (IP:17445-1-AP, 4ug; Detection:17445-1-AP 1:500) with mouse brain tissue lysate 5200ug.

  • IHC staining of human colon using 17445-1-AP

    IHC staining of human colon using 17445-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17445-1-AP (Oligophrenin 1 antibody at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon using 17445-1-AP

    IHC staining of human colon using 17445-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17445-1-AP (Oligophrenin 1 antibody at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse brain tissue, mouse skeletal muscle tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman colon tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

17445-1-AP targets Oligophrenin 1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen Oligophrenin 1 fusion protein Ag11538 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name oligophrenin 1
Synonyms MRX60, oligophrenin 1, OPHN1, OPN1
Calculated Molecular Weight 802 aa, 92 kDa
Observed Molecular Weight 91 kDa
GenBank Accession NumberBC059393
Gene Symbol Oligophrenin 1
Gene ID (NCBI) 4983
RRIDAB_2158304
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60890
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The OPHN1 gene encodes a Rho-GTPase activating protein (RhoGAP), and mutations in OPHN1 are responsible for non-specific X-linked mental retardation (NSMR). OPHN1 is highly expressed in the brain and present both pre- and postsynaptically in neurons.

实验方案

Product Specific Protocols
WB protocol for Oligophrenin 1 antibody 17445-1-APDownload protocol
IHC protocol for Oligophrenin 1 antibody 17445-1-APDownload protocol
IP protocol for Oligophrenin 1 antibody 17445-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Rep

Error-prone protein synthesis recapitulates early symptoms of Alzheimer disease in aging mice

Authors - Margarita Brilkova
View Article