验证数据展示
产品信息
80912-4-PBS targets Osteopontin in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, FC (Intra), Indirect ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | Osteopontin fusion protein Ag19216 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | secreted phosphoprotein 1 |
Synonyms | OPN, SPP1, SPP1, OPN, ETA 1, BSPI |
Calculated Molecular Weight | 314 aa, 35 kDa |
Observed Molecular Weight | 70 kDa, 44-66 kDa |
GenBank Accession Number | BC007016 |
Gene Symbol | Osteopontin |
Gene ID (NCBI) | 6696 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P10451-5 |
Storage Buffer | PBS Only |
Storage Conditions | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone, OPN has been found in kidneys, vascular tissues, biological fluids, and various tumor tissues (PMID: 15138464; 16406521). OPN can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression. The very acidic nature of OPN, as well as the presence of variable posttranslational modifications, has led to anomalous migration in SDS-polyacrylamide gels and therefore to reports of different molecular weights for OPN (PMID: 8293561). Depending on the cell and tissue source and/or the SDS-PAGE system, OPN migrates with a molecular weight of 44-80 kDa, as well as at some smaller bands corresponding to peptide fragments (PMID: 8195113; 17890765).