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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 80912-4-RR (same clone as 80912-4-PBS)

    WB analysis using 80912-4-RR (same clone as 80912-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80912-4-RR (OPN, SPP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80912-4-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 80912-4-RR (same clone as 80912-4-PBS)

    FC experiment of HepG2 using 80912-4-RR (same clone as 80912-4-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug OPN, SPP1 Recombinant antibody (80912-4-RR, Clone:6B11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80912-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80912-4-RR

    Affinity and Kinetic Characterization of 80912-4-RR

    Biolayer interferometry (BLl) kinetic assays of 80912-4-RR against Human OPN, SPP1 were performed. The affinity constant is 199 nM.

Osteopontin Recombinant antibody, PBS Only

Osteopontin Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 80912-4-PBS

产品说明书

CloneNo. 6B11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), Indirect ELISA

别名

OPN, SPP1, SPP1, 6B11, BNSP, Bone sialoprotein 1

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy Osteopontin/SPP1 Ready-To-Use IHC Kit. Osteopontin/SPP1 primary antibody included.

产品信息

80912-4-PBS targets Osteopontin in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Osteopontin fusion protein Ag19216 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 secreted phosphoprotein 1
别名 OPN, SPP1, SPP1, 6B11, BNSP, Bone sialoprotein 1
计算分子量 314 aa, 35 kDa
观测分子量 70 kDa, 44-66 kDa
GenBank蛋白编号BC007016
基因名称 Osteopontin
Gene ID (NCBI) 6696
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP10451-5
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone, OPN has been found in kidneys, vascular tissues, biological fluids, and various tumor tissues (PMID: 15138464; 16406521). OPN can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression. The very acidic nature of OPN, as well as the presence of variable posttranslational modifications, has led to anomalous migration in SDS-polyacrylamide gels and therefore to reports of different molecular weights for OPN (PMID: 8293561). Depending on the cell and tissue source and/or the SDS-PAGE system, OPN migrates with a molecular weight of 44-80 kDa, as well as at some smaller bands corresponding to peptide fragments (PMID: 8195113; 17890765).