Osteopontin Recombinant antibody
Osteopontin Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
6B11
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HepG2 cells, C2C12 cells, mouse kidney tissue, rat kidney tissue |
Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
产品信息
80912-4-RR targets Osteopontin in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, FC (Intra), ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Immunogen | Osteopontin fusion protein Ag19216 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | secreted phosphoprotein 1 |
Synonyms | OPN, SPP1, SPP1, OPN, ETA 1, BSPI |
Calculated Molecular Weight | 314 aa, 35 kDa |
Observed Molecular Weight | 70 kDa, 44-66 kDa |
GenBank Accession Number | BC007016 |
Gene Symbol | Osteopontin |
Gene ID (NCBI) | 6696 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P10451-5 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Osteopontin antibody 80912-4-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Death Dis LINC01133 promotes pancreatic ductal adenocarcinoma epithelial-mesenchymal transition mediated by SPP1 through binding to Arp3 | ||
J Neurooncol Exploring the prognostic value of BRMS1 + microglia based on single-cell anoikis regulator patterns in the immunologic microenvironment of GBM |