Osteopontin Recombinant antibody
Osteopontin Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IF/ICC, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
240206A2
验证数据展示
经过测试的应用
Positive WB detected in | MOLT-4 cells, HepG2 cells, HEK-293 cells |
Positive IF/ICC detected in | RAW 264.7 cells |
Positive FC (Intra) detected in | U-937 cells, A549 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 83341-1-RR is Osteopontin Fusion Protein expressed in E. coli.
Tested Applications | WB, IF/ICC, FC (Intra), ELISA Application Description |
Tested Reactivity | human, mouse |
Immunogen | Fusion Protein 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | secreted phosphoprotein 1 |
Synonyms | OPN, SPP1, SPP1, OPN, ETA 1, BSPI |
Calculated Molecular Weight | 314 aa, 35 kDa |
Observed Molecular Weight | 70 kDa, 44-66 kDa |
GenBank Accession Number | BC007016 |
Gene Symbol | Osteopontin |
Gene ID (NCBI) | 6696 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P10451-5 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Osteopontin antibody 83341-1-RR | Download protocol |
IF protocol for Osteopontin antibody 83341-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |