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验证数据展示

  • IHC staining of mouse eye using 24660-1-AP

    IHC staining of mouse eye using 24660-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 24660-1-AP (OPN1SW antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse eye using 24660-1-AP

    IF Staining of mouse eye using 24660-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using OPN1SW antibody (24660-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse eye using 24660-1-AP

    IF Staining of mouse eye using 24660-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using OPN1SW antibody (24660-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

OPN1SW Polyclonal antibody

OPN1SW Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 24660-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF-P, ELISA

别名

Blue sensitive opsin, Blue-sensitive opsin, BOP, CBT, Short-wave-sensitive opsin 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse eye tissue

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24660-1-AP targets OPN1SW in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 OPN1SW fusion protein Ag20387 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 opsin 1 (cone pigments), short-wave-sensitive
别名 Blue sensitive opsin, Blue-sensitive opsin, BOP, CBT, Short-wave-sensitive opsin 1
计算分子量 348 aa, 39 kDa
GenBank蛋白编号BC156719
基因名称 OPN1SW
Gene ID (NCBI) 611
RRIDAB_3085746
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP03999
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OPN1SW (Short-wave-sensitive opsin 1) belongs to the G-protein coupled receptor 1 family, opsin subfamily. It's one of three types of cone photoreceptors responsible for normal color vision. Inherited tritan color vision deficiencies exhibit an autosomal dominant inheritance pattern and are caused by mutations in OPN1SW (PMID: 32400513).

实验方案

Product Specific Protocols
IHC protocol for OPN1SW antibody 24660-1-APDownload protocol
IF protocol for OPN1SW antibody 24660-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols