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验证数据展示

  • IHC staining of mouse eye using 83754-6-RR

    IHC staining of mouse eye using 83754-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 83754-6-RR (OPN1SW antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse eye using 83754-6-RR

    IF Staining of mouse eye using 83754-6-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using OPN1SW antibody (83754-6-RR, Clone: 240714A5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83754-6-RR

    Affinity and Kinetic Characterization of 83754-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83754-6-RR against Human OPN1SW were performed. The affinity constant is 3.58 nM.

OPN1SW Recombinant antibody

OPN1SW Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 83754-6-RR

产品说明书

CloneNo. 240714A5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, ELISA

别名

Short-wave-sensitive opsin 1, CBT, BOP, Blue-sensitive opsin, Blue sensitive opsin

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inmouse eye tissue

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83754-6-RR targets OPN1SW in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 OPN1SW fusion protein Ag20445 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 opsin 1 (cone pigments), short-wave-sensitive
别名 Short-wave-sensitive opsin 1, CBT, BOP, Blue-sensitive opsin, Blue sensitive opsin
计算分子量 348 aa, 39 kDa
GenBank蛋白编号BC156719
基因名称 OPN1SW
Gene ID (NCBI) 611
RRIDAB_3671351
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP03999
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OPN1SW (Short-wave-sensitive opsin 1) belongs to the G-protein coupled receptor 1 family, opsin subfamily. It's one of three types of cone photoreceptors responsible for normal color vision. Inherited tritan color vision deficiencies exhibit an autosomal dominant inheritance pattern and are caused by mutations in OPN1SW (PMID: 32400513).

实验方案

Product Specific Protocols
IHC protocol for OPN1SW antibody 83754-6-RRDownload protocol
IF protocol for OPN1SW antibody 83754-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols