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OPTN Recombinant antibody, PBS Only (Capture)

OPTN Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240636E8

Cat no : 83634-2-PBS

Print datasheet

Synonyms

Optineurin, FIP2, FIP 2, FAA4, E3-14.7K-interacting protein

验证数据展示

  • Sandwich ELISA standard curve of MP00621-2

    Sandwich ELISA standard curve of MP00621-2

    Sandwich ELISA standard curve of MP00621-2, Human OPTN Recombinant Matched Antibody Pair - PBS only. 83634-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1272. 83634-3-PBS was HRP conjugated as the detection antibody. Range: 93.8-6000 pg/mL

  • Sample test of MP00621-2

    Sample test of MP00621-2

    Jurkat and MCF-7 cell lysates were measured. The human OPTN concentration of detected samples was determined to be 29,772.0 pg/mL (based on a 1.0 mg/mL extract load) in Jurkat cell lysate and 8,643.8 pg/mL (based on a 4.3 mg/mL extract load) in MCF-7 cell lysate.

  • Sample test of MP00621-2

    Sample test of MP00621-2

    Serum of sixteen individual healthy human donors was measured. The human OPTN concentration of detected samples was determined to be 813.0 pg/mL with a range of 92.0 - 5,037.9 pg/mL.

  • Cytometric bead array standard curve of MP00621-2

    Cytometric bead array standard curve of MP00621-2

    Cytometric bead array standard curve of MP00621-2, OPTN Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83634-2-PBS. Detection antibody: 83634-3-PBS. Standard: Ag1272. Range: 1.25-80 ng/mL.

  • Cytometric bead array standard curve of MP00621-3

    Cytometric bead array standard curve of MP00621-3

    Cytometric bead array standard curve of MP00621-3, OPTN Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83634-2-PBS. Detection antibody: 83634-1-PBS. Standard: Ag1272. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 83634-2-PBS

    Affinity and Kinetic Characterization of 83634-2-PBS

    Biolayer interferometry (BLl) kinetic assay of 83634-2-PBS against Human OPTN was performed. The affinity constant is below 1 pM.

产品信息

83634-2-PBS targets OPTN as part of a matched antibody pair:

MP00621-2: 83634-2-PBS capture and 83634-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00621-3: 83634-2-PBS capture and 83634-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen OPTN fusion protein Ag1272 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name optineurin
Synonyms Optineurin, FIP2, FIP 2, FAA4, E3-14.7K-interacting protein
Calculated Molecular Weight 66 kDa
GenBank Accession NumberBC013876
Gene Symbol OPTN
Gene ID (NCBI) 10133
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96CV9
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C