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Species Reactivity
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0
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0
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0
Conjugate
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验证数据展示

  • WB analysis using 83634-5-RR (same clone as 83634-5-PBS)

    WB analysis using 83634-5-RR (same clone as 83634-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83634-5-RR (OPTN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83634-5-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 83634-5-RR (same clone as 83634-5-PBS)

    IHC staining of human gliomas using 83634-5-RR (same clone as 83634-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 83634-5-RR (OPTN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83634-5-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 83634-5-RR (same clone as 83634-5-PBS)

    IHC staining of human gliomas using 83634-5-RR (same clone as 83634-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 83634-5-RR (OPTN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83634-5-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83634-5-RR (same clone as 83634-5-PBS)

    FC experiment of HeLa using 83634-5-RR (same clone as 83634-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug OPTN Recombinant antibody (83634-5-RR, Clone:240636A7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83634-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83634-5-RR

    Affinity and Kinetic Characterization of 83634-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83634-5-RR against Human OPTN were performed. The affinity constant is 12.2 nM.

OPTN/Optineurin Recombinant antibody, PBS Only

OPTN/Optineurin Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83634-5-PBS

产品说明书

CloneNo. 240636A7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), ELISA

别名

Optineurin, OPTN, FIP2, FIP 2, FAA4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83634-5-PBS targets OPTN/Optineurin in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 OPTN/Optineurin fusion protein Ag1272 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 optineurin
别名 Optineurin, OPTN, FIP2, FIP 2, FAA4
计算分子量 66 kDa
观测分子量 66-70 kDa
GenBank蛋白编号BC013876
基因名称 OPTN
Gene ID (NCBI) 10133
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ96CV9
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

OPTN, also named as FIP2, GLC1E, HIP7, HYPL, and NRP, plays a neuroprotective role in the eye and optic nerve. It is probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. OPTN may act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). OPTN may constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. Defects in OPTN cause susceptibility to normal pressure glaucoma (NPG). OPTN mutated in adult-onset primary open angle glaucoma. It supports the protective role of OPTN in the trabecular meshwork.