验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, mouse ovary tissue, PC-3 cells, A375 cells, mouse spleen tissue |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13130-1-AP targets ORAI1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, zebrafish |
| 免疫原 | ORAI1 fusion protein Ag3758 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ORAI calcium release-activated calcium modulator 1 |
| 别名 | CRACM1, ORAI1 L1, ORAI1 L2, Orail L2, ORAT1, Protein orai 1, TMEM142A, Transmembrane protein 142A |
| 计算分子量 | 301 aa, 33 kDa |
| 观测分子量 | 45-50 kDa |
| GenBank蛋白编号 | BC015369 |
| 基因名称 | ORAI1 |
| Gene ID (NCBI) | 84876 |
| RRID | AB_2157558 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96D31 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Store-operated calcium channels (SOCs) play essential functions in various physiological processes. Orai1 (also named CRACM1) is a critical molecular component of SOCs. Orai1 protein is a 32-55 kDa plasma membrane protein with 4 transmembrane domains. Human patients with a severe combinaed immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ORAI1 antibody 13130-1-AP | Download protocol |
| IF protocol for ORAI1 antibody 13130-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Res Direct control of store-operated calcium channels by ultrafast laser.
| ||
Blood Orai1 (CRACM1) is the platelet SOC channel and essential for pathological thrombus formation. | ||
Blood The serum- and glucocorticoid-inducible kinase 1 (SGK1) influences platelet calcium signaling and function by regulation of Orai1 expression in megakaryocytes. | ||
Kidney Int AMP-activated kinase is a regulator of fibroblast growth factor 23 production. | ||
Sci Signal Adhesion to laminin-1 and collagen IV induces the formation of Ca2+ microdomains that sensitize mouse T cells for activation | ||
Sci Signal ORAI1, STIM1/2, and RYR1 shape subsecond Ca2+ microdomains upon T cell activation. |
