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ORAI1 Polyclonal antibody

ORAI1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 14442-1-AP

Print datasheet

Synonyms

CRACM1, ORAI1, ORAI1 L1, ORAI1 L2, Orail L2, ORAT1, Protein orai 1, TMEM142A, Transmembrane protein 142A

验证数据展示

  • WB analysis using 14442-1-AP

    WB analysis using 14442-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14442-1-AP (ORAI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inhuman peripheral blood platelets, mouse skeletal muscle tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IPSee 1 publications below

产品信息

14442-1-AP targets ORAI1 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsIP
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ORAI calcium release-activated calcium modulator 1
Synonyms CRACM1, ORAI1, ORAI1 L1, ORAI1 L2, Orail L2, ORAT1, Protein orai 1, TMEM142A, Transmembrane protein 142A
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 30-35 kDa, 45-55 kDa
GenBank Accession NumberNM_032790
Gene Symbol ORAI1
Gene ID (NCBI) 84876
RRIDAB_2157435
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96D31
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Store-operated calcium channels (SOCs) play essential functions in various physiological processes. Orai1 (also named CRACM1) is a critical molecular component of SOCs. Orai1 protein is a ~35 kDa plasma membrane protein with 4 transmembrane domains. ORAI1 is a complex glycosylated protein, it exists in several molecular weight forms: a theoretical molecular weight of 32.7 kDa of the unmodified protein and two major glycosylated proteins of ~43 kDa and ~50 kDa (PMID: 22318387). Human patients with a severe combined immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene. This antibody may be used to block the ion currents mediated by Orai1.

实验方案

Product Specific Protocols
WB protocol for ORAI1 antibody 14442-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIP

BMJ Open Diabetes Res Care

Orai-vascular endothelial-cadherin signaling complex regulates high-glucose exposure-induced increased permeability of mouse aortic endothelial cells.

Authors - Yuan Wei
View Article