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ORC5L Polyclonal antibody

ORC5L Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11542-1-AP

Print datasheet

Synonyms

ORC5T, ORC5P, ORC5

验证数据展示

  • IF Staining of HepG2 using 11542-1-AP

    IF Staining of HepG2 using 11542-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11542-1-AP (ORC5L antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human pancreas cancer using 11542-1-AP

    IHC staining of human pancreas cancer using 11542-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11542-1-AP (ORC5L antibody) at dilution of 1:50 (under 10x lens).

  • WB analysis of mouse kidney using 11542-1-AP

    WB analysis of mouse kidney using 11542-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11542-1-AP (ORC5L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse kidney tissue, human kidney tissue, mouse ovary tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 2 publications below

产品信息

11542-1-AP targets ORC5L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen ORC5L fusion protein Ag2133 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name origin recognition complex, subunit 5-like (yeast)
Synonyms ORC5T, ORC5P, ORC5
Calculated Molecular Weight 435 aa, 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC023652
Gene Symbol ORC5L
Gene ID (NCBI) 5001
RRIDAB_2267695
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43913
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Origin recognition complex (ORC) plays an essential role in the initiation of chromosome DNA replication and transcriptional silencing. ORC binds in an ATP-dependent manner to several well-defined autonomously replicating sequences that serve as the chromosomal replication origins. ORC5L is one of the 6 subunits of ORC.

实验方案

Product Specific Protocols
WB protocol for ORC5L antibody 11542-1-APDownload protocol
IHC protocol for ORC5L antibody 11542-1-APDownload protocol
IF protocol for ORC5L antibody 11542-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Cancer Med

Systemic analysis of the DNA replication regulator origin recognition complex in lung adenocarcinomas identifies prognostic and expression significance

Authors - Min Tang
View Article
humanIHC

Oncol Lett

PSMC2, ORC5 and KRTDAP are specific biomarkers for HPV-negative head and neck squamous cell carcinoma.

Authors - Yushen Su
View Article