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验证数据展示

  • WB analysis using 82932-1-RR (same clone as 82932-1-PBS)

    WB analysis using 82932-1-RR (same clone as 82932-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82932-1-RR (OS9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82932-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 82932-1-RR (same clone as 82932-1-PBS)

    IHC staining of mouse testis using 82932-1-RR (same clone as 82932-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82932-1-RR (OS9 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82932-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 82932-1-RR (same clone as 82932-1-PBS)

    IHC staining of mouse testis using 82932-1-RR (same clone as 82932-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82932-1-RR (OS9 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82932-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 82932-1-RR (same clone as 82932-1-PBS)

    FC experiment of HeLa using 82932-1-RR (same clone as 82932-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug OS9 Recombinant antibody (82932-1-RR, Clone:230149B4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82932-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82932-1-RR

    Affinity and Kinetic Characterization of 82932-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82932-1-RR against Human OS9 were performed. The affinity constant is below 1 pM.

OS9 Recombinant antibody, PBS Only

OS9 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 82932-1-PBS

产品说明书

CloneNo. 230149B4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

Protein OS-9, Amplified in osteosarcoma 9, 230149B4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

82932-1-PBS targets OS9 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 OS9 fusion protein Ag0106 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 osteosarcoma amplified 9, endoplasmic reticulum associated protein
别名 Protein OS-9, Amplified in osteosarcoma 9, 230149B4
计算分子量 76 kDa
观测分子量83-97 kDa
GenBank蛋白编号BC000532
基因名称 OS9
Gene ID (NCBI) 10956
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13438
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

OS-9 is a ubiquitously expressed ensoplasmic reticulum (ER)-associated protein originally identified as being amplified in certain osteosarcomas. It functions in ER quality control and ER-associated degradation (ERAD). There are three isoforms of this protein: OS-9.1, OS-9.2 and OS-9.3. The longest isoform, OS-9.1, contains 667 aa, OS-9.2 lacks aa 535-589, whereas OS-9.3 lacks aa 456-470 and 535-589. OS-9.1 and OS-9.2 are N-glycosylated, ubiquitously expressed in human tissues, and amplified in tumors.