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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 82932-1-RR (same clone as 82932-1-PBS)

    WB analysis using 82932-1-RR (same clone as 82932-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82932-1-RR (OS9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82932-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 82932-1-RR (same clone as 82932-1-PBS)

    IHC staining of mouse testis using 82932-1-RR (same clone as 82932-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82932-1-RR (OS9 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82932-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 82932-1-RR (same clone as 82932-1-PBS)

    IHC staining of mouse testis using 82932-1-RR (same clone as 82932-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82932-1-RR (OS9 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82932-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 82932-1-RR (same clone as 82932-1-PBS)

    FC experiment of HeLa using 82932-1-RR (same clone as 82932-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug OS9 Recombinant antibody (82932-1-RR, Clone:230149B4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82932-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82932-1-RR

    Affinity and Kinetic Characterization of 82932-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82932-1-RR against Human OS9 were performed. The affinity constant is below 1 pM.

OS9 Recombinant antibody, PBS Only

OS9 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 82932-1-PBS
CloneNo. 230149B4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

Protein OS-9, Amplified in osteosarcoma 9, 230149B4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

82932-1-PBS targets OS9 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen OS9 fusion protein Ag0106 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name osteosarcoma amplified 9, endoplasmic reticulum associated protein
Synonyms Protein OS-9, Amplified in osteosarcoma 9, 230149B4
Calculated Molecular Weight 76 kDa
Observed Molecular Weight83-97 kDa
GenBank Accession NumberBC000532
Gene Symbol OS9
Gene ID (NCBI) 10956
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13438
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

OS-9 is a ubiquitously expressed ensoplasmic reticulum (ER)-associated protein originally identified as being amplified in certain osteosarcomas. It functions in ER quality control and ER-associated degradation (ERAD). There are three isoforms of this protein: OS-9.1, OS-9.2 and OS-9.3. The longest isoform, OS-9.1, contains 667 aa, OS-9.2 lacks aa 535-589, whereas OS-9.3 lacks aa 456-470 and 535-589. OS-9.1 and OS-9.2 are N-glycosylated, ubiquitously expressed in human tissues, and amplified in tumors.