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OSBPL11 Polyclonal antibody

OSBPL11 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11318-1-AP

Print datasheet

Synonyms

ORP 11, ORP11, OSBP related protein 11, OSBP12, OSBPL11, TCCCIA00292

验证数据展示

  • WB analysis using 11318-1-AP

    WB analysis using 11318-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11318-1-AP (OSBPL11 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 11318-1-AP

    IHC staining of human ovary tumor using 11318-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11318-1-AP (OSBPL11 antibody) at dilution of 1:50 (under 10x lens).

  • IP experiment of mouse testis using 11318-1-AP

    IP experiment of mouse testis using 11318-1-AP

    IP result of anti-OSBPL11 (IP:11318-1-AP, 4ug; Detection:11318-1-AP 1:300) with mouse testis tissue lysate 4000ug.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11318-1-AP targets OSBPL11 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen OSBPL11 fusion protein Ag1855 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name oxysterol binding protein-like 11
Synonyms ORP 11, ORP11, OSBP related protein 11, OSBP12, OSBPL11, TCCCIA00292
Calculated Molecular Weight 747 aa, 84 kDa
Observed Molecular Weight 84 kDa
GenBank Accession NumberBC010422
Gene Symbol OSBPL11
Gene ID (NCBI) 114885
RRIDAB_2267735
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BXB4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for OSBPL11 antibody 11318-1-APDownload protocol
IHC protocol for OSBPL11 antibody 11318-1-APDownload protocol
IP protocol for OSBPL11 antibody 11318-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Elife

The ORP9-ORP11 dimer promotes sphingomyelin synthesis

Authors - Birol Cabukusta
View Article