验证数据展示
经过测试的应用
| Positive WB detected in | PC-3 cells, human heart tissue, Raji cells, mouse colon tissue |
| Positive IHC detected in | human kidney tissue, human heart tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy OSGEP Ready-To-Use IHC Kit. OSGEP primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
15033-1-AP targets OSGEP in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | OSGEP fusion protein Ag4308 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | O-sialoglycoprotein endopeptidase |
| 别名 | GCPL1, hOSGEP, KAE1, OSGEP, OSGEP1, PRSMG1 |
| 计算分子量 | 36 kDa |
| 观测分子量 | 36 kDa |
| GenBank蛋白编号 | BC032310 |
| 基因名称 | OSGEP |
| Gene ID (NCBI) | 55644 |
| RRID | AB_2283171 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NPF4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
OSGEP(O-sialoglycoprotein endopeptidase) is also named as hOSGEP, GCPL1 and belongs to the KAE1 / YgjD family. The deduced 335-amino acid OSGEP protein has a predicted molecular mass of 36.4 kDa. It is a protease whose target is O-linked sialic acid-containing glycoproteins, such as episialin(PMID:10859271). It is involved in the metabolism of glycated proteins as a glycoprotein-related chaperon protease(PMID: 20824107).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for OSGEP antibody 15033-1-AP | Download protocol |
| IHC protocol for OSGEP antibody 15033-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
