• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

OSGEPL1 Polyclonal antibody

OSGEPL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25694-1-AP

Print datasheet

Synonyms

OSGEPL1

验证数据展示

  • WB analysis of HeLa using 25694-1-AP

    WB analysis of HeLa using 25694-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25694-1-AP (OSGEPL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 25694-1-AP

    IHC staining of human colon using 25694-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25694-1-AP (OSGEPL1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon using 25694-1-AP

    IHC staining of human colon using 25694-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25694-1-AP (OSGEPL1 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, NIH/3T3 cells
Positive IHC detected inhuman colon tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

25694-1-AP targets OSGEPL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen OSGEPL1 fusion protein Ag22204 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name O-sialoglycoprotein endopeptidase-like 1
Synonyms OSGEPL1
Calculated Molecular Weight 414 aa, 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC011904
Gene Symbol OSGEPL1
Gene ID (NCBI) 64172
RRIDAB_2880198
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H4B0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for OSGEPL1 antibody 25694-1-APDownload protocol
IHC protocol for OSGEPL1 antibody 25694-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

CO2-sensitive tRNA modification associated with human mitochondrial disease.

Authors - Huan Lin
View Article