oncostatin M Polyclonal antibody

oncostatin M Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 27792-1-AP

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Synonyms

OSM, Oncostatin-M



经过测试的应用

Positive WB detected inMCF-7 cells, Jurkat cells, rat testis tissue
Positive IHC detected inhuman colon cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27792-1-AP targets oncostatin M in WB, IHC, IF, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC, IF
Tested Reactivity human, rat
Cited Reactivityhuman, mouse
Immunogen oncostatin M fusion protein Ag27093 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name oncostatin M
Synonyms OSM, Oncostatin-M
Calculated Molecular Weight 252 aa, 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC011589
Gene Symbol OSM
Gene ID (NCBI) 5008
RRIDAB_2880974
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13725
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Oncostatin M (OSM) is a multifunctional cytokine that belongs to the interleukin-6 (IL-6) family. It is primarily secreted by activated T lymphocytes and macrophages as a 28 kDa glycoprotein. OSM shares properties with all members of this cytokine family, but is most closely related in structure and function to leukemia inhibitory factor (LIF). OSM acts on a variety of cells and is involved in regulating gene activation, cell growth, and inflammatory processes. It is a potent inducer of anti-proteases, has anti-inflammatory properties, acts as an immune modifier, and promotes the deposition of extracellular matrix proteins . In the context of respiratory diseases, OSM may play a significant role in normal wound healing, as well as in remodeling and pathological fibrosis.

实验方案

Product Specific Protocols
WB protocol for oncostatin M antibody 27792-1-APDownload protocol
IHC protocol for oncostatin M antibody 27792-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Jpn J Ophthalmol

Role of oncostatin M in the pathogenesis of vernal keratoconjunctivitis: focus on tissue remodeling.

Authors - Keitaro Mashimo
mouseIHC

Int J Radiat Oncol Biol Phys

Oncostatin M/Oncostatin M Receptor Signal Induces Radiation-Induced Heart Fibrosis by Regulating SMAD4 in Fibroblast

Authors - Peng Xu