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验证数据展示

  • WB analysis using 30606-1-AP

    WB analysis using 30606-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30606-1-AP (OSR1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse embryo using 30606-1-AP

    IHC staining of mouse embryo using 30606-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 30606-1-AP (OSR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

OSR1 Polyclonal antibody

OSR1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30606-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Protein odd-skipped-related 1, Protein odd skipped related 1, ODD

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse embryo tissue, rat bladder tissue
Positive IHC detected inmouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30606-1-AP targets OSR1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 OSR1 fusion protein Ag31336 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 odd-skipped related 1 (Drosophila)
别名 Protein odd-skipped-related 1, Protein odd skipped related 1, ODD
计算分子量 30 kDa
观测分子量 30 kDa
GenBank蛋白编号BC025712
基因名称 OSR1
Gene ID (NCBI) 130497
RRIDAB_3669737
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TAX0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Odd-skipped-related1(OSR1), contains 3 C2H3-type zinc fingers, a tyrosine phosphorylation site and several putative PXXP SH3 binding motifs. It's a transcription factor that has a role in embryonic heart and urogenital development. Expressed during the beginning stages of embryogenesis and during limb and branchial arch development, it possibly participarts in the metanephric kidney formation. OSR1 also plays a key role in the generation of kidney precursor cells and their subsequent differentiation into nephrons. OSR1 is upregulated in several pancreatic and esopha-geal cancer cell lines and downregulated in some primary gastric cancers

实验方案

Product Specific Protocols
WB protocol for OSR1 antibody 30606-1-APDownload protocol
IHC protocol for OSR1 antibody 30606-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols