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OSTM1 Polyclonal antibody

OSTM1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14621-1-AP

Print datasheet

Synonyms

UNQ6098/PRO21201, Osteopetrosis-associated transmembrane protein 1, HSPC019, GL, Chloride channel 7 beta subunit

验证数据展示

  • WB analysis using 14621-1-AP

    WB analysis using 14621-1-AP

    mouse brain membrane protein lysates were subjected to SDS PAGE followed by western blot with 14621-1-AP (OSTM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 14621-1-AP

    IHC staining of mouse kidney using 14621-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14621-1-AP (OSTM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 14621-1-AP

    IHC staining of mouse kidney using 14621-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14621-1-AP (OSTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 14621-1-AP

    IF Staining of A431 using 14621-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using OSTM1 antibody (14621-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected in37°C incubated mouse brain tissue, mouse brain membrane protein
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

14621-1-AP targets OSTM1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen OSTM1 fusion protein Ag6254 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name osteopetrosis associated transmembrane protein 1
Synonyms UNQ6098/PRO21201, Osteopetrosis-associated transmembrane protein 1, HSPC019, GL, Chloride channel 7 beta subunit
Calculated Molecular Weight 37 kDa
Observed Molecular Weight~ 60 kDa
GenBank Accession NumberBC068581
Gene Symbol OSTM1
Gene ID (NCBI) 28962
RRIDAB_2298955
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86WC4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OSTM1 is a type I transmembrane protein. Ostm1 is essential for osteoclast maturation and is responsible for the most severe forms of osteoporosis when mutated in mice and humans. The OSTM1 protein has 10 N-glycosylation sites and has an apparent mass of ~60 kDa after being highly N-glycosylated (PMID: 26598607; PMID: 32764302). The OSTM1 protein can also be detected at ~30-45 kDa and ~70-80 kDa in some literatures (PMID: 16525474; PMID: 24159188;PMID: 20830208).

实验方案

Product Specific Protocols
WB protocol for OSTM1 antibody 14621-1-APDownload protocol
IHC protocol for OSTM1 antibody 14621-1-APDownload protocol
IF protocol for OSTM1 antibody 14621-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Virol

Osteopetrosis-Associated Transmembrane Protein 1 Recruits RNA Exosome To Restrict Hepatitis B Virus Replication.

Authors - Chunqiang Ma
View Article
mouseWB

Biology (Basel)

Leucine Repeat Rich Kinase 1 Controls Osteoclast Activity by Managing Lysosomal Trafficking and Secretion

Authors - Sandi Shen
View Article