验证数据展示
经过测试的应用
| Positive WB detected in | pig liver tissue, rat liver tissue, mouse liver tissue |
| Positive IHC detected in | human liver cancer tissue, human liver tissue, rat colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy OTC Ready-To-Use IHC Kit. OTC primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:4000-1:16000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
67553-1-Ig targets OTC in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, mouse, rat, pig |
| 文献引用反应性 | human, mouse |
| 免疫原 | OTC fusion protein Ag24186 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ornithine carbamoyltransferase |
| 别名 | OCTD, ornithine carbamoyltransferase, Ornithine transcarbamylase, OTC, OTCase |
| 计算分子量 | 354 aa, 40 kDa |
| 观测分子量 | 36 kDa |
| GenBank蛋白编号 | BC074745 |
| 基因名称 | OTC |
| Gene ID (NCBI) | 5009 |
| RRID | AB_2882767 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P00480 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ornithine transcarbamylase (OTC) is a liver and intestinal mucosa-located, intramitochondrial, rate-limiting enzyme in the urea cycle. It catalyzes the reaction that converts ornithine and carbamoyl phosphate into citrulline, which is the second step of the urea cycle, to detoxify the ammonia produced from amino acid catabolism.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for OTC antibody 67553-1-Ig | Download protocol |
| IHC protocol for OTC antibody 67553-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Transl Med Aberrant methylmalonylation underlies methylmalonic acidemia and is attenuated by an engineered sirtuin | ||
Biomater Res Genetically Modified Hepatocytes Targeting Bilirubin and Ammonia Metabolism for the Construction of Bioartificial Liver System |
