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验证数据展示

  • IHC staining of rat brain using 20721-1-AP

    IHC staining of rat brain using 20721-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20721-1-AP (OTOF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 20721-1-AP

    IHC staining of rat brain using 20721-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20721-1-AP (OTOF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

OTOF Polyclonal antibody

OTOF Polyclonal Antibody for IHC, ELISA
Cat No. 20721-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, ELISA

别名

DFNB6, DFNB9, Fer 1 like protein 2, FER1L2, NSRD9, OTOF, otoferlin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inrat brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20721-1-AP targets OTOF in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 otoferlin
别名 DFNB6, DFNB9, Fer 1 like protein 2, FER1L2, NSRD9, OTOF, otoferlin
计算分子量 227 kDa
GenBank蛋白编号NM_004802
基因名称 OTOF
Gene ID (NCBI) 9381
RRIDAB_3085618
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HC10
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Otoferlin, a C2 domain transmembrane protein, is a major calcium sensor regulating extracellular secretion in mammalian internal hair cells. Deafness caused by mutations in the OTOF gene encoding Otoferlin is a recessive genetic disorder, a sensorineural deafness. It is expressed in the brain, inner hair cells and vestibular hair cells.(PMID: 17055430; PMID: 17229086)

实验方案

Product Specific Protocols
IHC protocol for OTOF antibody 20721-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols